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Effect of light scattering on optical-resolution photoacoustic microscopy

Liu, Yan and Zhang, Chi and Hu, Song and Suzuki, Yuta and Xu, Zhun and Ferradal, Silvina and Wang, Lihong V. (2012) Effect of light scattering on optical-resolution photoacoustic microscopy. In: Photons Plus Ultrasound: Imaging and Sensing 2012. Proceedings of SPIE. No.8223. Society of Photo-optical Instrumentation Engineers (SPIE) , Bellingham, WA, Art. No. 82233S. ISBN 9780819488664.

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The penetration depth of ballistic optical imaging technologies is limited by light scattering. To study the effect of scattering on optical-resolution photoacoustic microscopy (OR-PAM), we divided the signals in OR-PAM into two classes: one is from the target volume defined by the optical resolution cell (Class I); the other is from the rest of the acoustic resolution cell (Class II). We developed a way to simulate the point spread function (PSF) of our OR-PAM system considering both optical illumination and acoustic detection, then used the PSF to calculate the contributions of each class of signal to the total signal at different focal depths. Our simulation results showed that: 1) The Class II signal decays much more slowly than the Class I signal; 2) The full width at half maximum (FWHM) of the PSF for the focal depth of 0.9 transport mean free path (TMFP) is not broadened much (~10%) compared with that for a clear medium; 3) Image contrast is degraded with increasing depth when there is a uniform absorption background.

Item Type:Book Section
Related URLs:
URLURL TypeDescription
Liu, Yan0000-0002-5837-4908
Zhang, Chi0000-0002-1324-2311
Wang, Lihong V.0000-0001-9783-4383
Additional Information:© 2012 Society of Photo-Optical Instrumentation Engineers (SPIE). We would like to thank Zijian Guo and Kun Wang for helpful discussions. This work was sponsored in part by National Institutes of Health grants R01 EB008085, R01 EB000712, R01 CA134539, R01 CA157277, and U54 CA 136398. L. W. has a financial interest in Microphotoacoustics, Inc. and Endra, Inc., which, however, did not support this work.
Funding AgencyGrant Number
NIHR01 EB008085
NIHR01 EB000712
NIHR01 CA134539
NIHR01 CA157277
NIHU54 CA136398
Subject Keywords:Optical-resolution photoacoustic microscopy, light scattering, penetration depth
Series Name:Proceedings of SPIE
Issue or Number:8223
Record Number:CaltechAUTHORS:20180918-140709789
Persistent URL:
Official Citation:Yan Liu, Yan Liu, Chi Zhang, Chi Zhang, Song Hu, Song Hu, Yuta Suzuki, Yuta Suzuki, Zhun Xu, Zhun Xu, Silvina Ferradal, Silvina Ferradal, Lihong V. Wang, Lihong V. Wang, } "Effect of light scattering on optical-resolution photoacoustic microscopy", Proc. SPIE 8223, Photons Plus Ultrasound: Imaging and Sensing 2012, 82233S (23 February 2012); doi: 10.1117/12.909373;
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:89727
Deposited By: George Porter
Deposited On:19 Sep 2018 21:00
Last Modified:16 Nov 2021 00:37

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