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Double-illumination photoacoustic microscopy of intestinal hemodynamics following massive small bowel resection

Yao, Junjie and Rowland, Kathryn J. and Wang, Lidai and Maslov, Konstantin I. and Warner, Brad W. and Wang, Lihong V. (2012) Double-illumination photoacoustic microscopy of intestinal hemodynamics following massive small bowel resection. In: Photons Plus Ultrasound: Imaging and Sensing 2012. Proceedings of SPIE. No.8223. Society of Photo-optical Instrumentation Engineers (SPIE) , Bellingham, WA, Art. No. 82233V. ISBN 9780819488664. https://resolver.caltech.edu/CaltechAUTHORS:20180918-140709935

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Abstract

Massive small bowel resection (SBR) results in villus angiogenesis and intestinal adaptation. The exact mechanism that causes intestinal villus angiogenesis remains unknown. We hypothesize that hemodynamic changes within the remnant bowel after SBR will trigger intestinal angiogenesis. To validate this, we used photoacoustic microscopy (PAM) to image the microvascular system of the intestine in C57B6 mice and to measure blood flow and oxygen saturation (sO_2) of a supplying artery and vein. Baseline measurements were made 6 cm proximal to the ileal-cecal junction (ICJ) prior to resection. A 50% proximal bowel resection was then performed, and measurements were again recorded at the same location immediately, 1, 3 and 7 days following resection. The results show that arterial and venous sO_2 were similar prior to SBR. Immediately following SBR, the arterial and venous sO_2 decreased by 14.3 ± 2.7% and 32.7 ± 6.6%, respectively, while the arterial and venous flow speed decreased by 62.9 ± 17.3% and 60.0 ± 20.1%, respectively. Such significant decreases in sO_2 and blood flow indicate a hypoxic state after SBR. Within one week after SBR, both sO2 and blood flow speed had gradually recovered. By 7 days after SBR, arterial and venous sO_2 had increased to 101.0 ± 2.9% and 82.7 ± 7.3% of the baseline values, respectively, while arterial and venous flow speed had increased to 106.0 ± 21.4% and 150.0 ± 29.6% of the baseline values, respectively. Such increases in sO_2 and blood flow may result from angiogenesis following SBR.


Item Type:Book Section
Related URLs:
URLURL TypeDescription
https://doi.org/10.1117/12.909508DOIArticle
ORCID:
AuthorORCID
Maslov, Konstantin I.0000-0003-3408-8840
Wang, Lihong V.0000-0001-9783-4383
Additional Information:© 2012 Society of Photo-Optical Instrumentation Engineers (SPIE). Wang laboratory research was supported by the National Institutes of Health Grants R01 EB000712, R01 EB008085, R01 CA134539, U54 CA136398, R01 EB010049, R01 CA157277, and 5P60 DK02057933. L.V.W. has a financial interest in Microphotoacoustics, Inc. and Endra, Inc., which, however, did not support this work. Warner laboratory research was support by National Institutes of Health Grants R01 DK059288 (Warner) and T32 CA009621 (Rowland). The authors thank Yan Liu and Joon-Mo Yang for useful discussion and technical assistance.
Funders:
Funding AgencyGrant Number
NIHR01 EB000712
NIHR01 EB008085
NIHR01 CA134539
NIHU54 CA136398
NIHR01 EB010049
NIHR01 CA157277
NIH5P60 DK02057933
NIHR01 DK059288
NIH Predoctoral FellowshipT32 CA009621
Subject Keywords:photoacoustic microscopy, small bowel resection, oxygen saturation, blood flow, hypoxia, angiogenesis
Series Name:Proceedings of SPIE
Issue or Number:8223
DOI:10.1117/12.909508
Record Number:CaltechAUTHORS:20180918-140709935
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20180918-140709935
Official Citation:Junjie Yao, Junjie Yao, Kathryn J. Rowland, Kathryn J. Rowland, Lidai Wang, Lidai Wang, Konstantin I. Maslov, Konstantin I. Maslov, Brad W. Warner, Brad W. Warner, Lihong V. Wang, Lihong V. Wang, } "Double-illumination photoacoustic microscopy of intestinal hemodynamics following massive small bowel resection", Proc. SPIE 8223, Photons Plus Ultrasound: Imaging and Sensing 2012, 82233V (23 February 2012); doi: 10.1117/12.909508; https://doi.org/10.1117/12.909508
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:89729
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:19 Sep 2018 21:19
Last Modified:16 Nov 2021 00:37

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