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Enzymatic synthesis of DNA, XXIV. Synthesis of infectious phage phiX174 DNA

Goulian, Mehran and Kornberg, Arthur and Sinsheimer, Robert L. (1967) Enzymatic synthesis of DNA, XXIV. Synthesis of infectious phage phiX174 DNA. Proceedings of the National Academy of Sciences of the United States of America, 58 (6). pp. 2321-2328. ISSN 0027-8424. PMCID PMC223838. http://resolver.caltech.edu/CaltechAUTHORS:GOUpnas67

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Abstract

Past attempts at in vitro replication of transforming factor present in DNA have given negative or inconclusive results.(1-3) Rigid proof was lacking that template material had been excluded from the synthetic product. Even if a rigorous demonstration of net synthesis of transforming factor for a given genetic marker were forthcoming, it would still prove only that some relatively short sequence of nucleotides, sufficient for replacement of the mutant locus, had been synthesized. If enzymatic synthesis of infectious bacteriophage DNA were achieved, it would be made clear at once that relatively few, if any, mistakes had been made in replicating a DNA sequence of several thousand nucleotides.


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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC223838/PubMed CentralArticle
Additional Information:© 1967 by the National Academy of Sciences. Communicated September 25, 1967. We gratefully acknowledge the expert assistance of Mrs. Gloria Davis of the Division of Biology at the California Institute of Technology in performing the spheroplast assays for infectivity. This research was supported by grants from the National Institutes of Health and the National Science Foundation. [M.G. was an] USPHS special fellow.
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U.S. Public Health Service (USPHS)UNSPECIFIED
Issue or Number:6
PubMed Central ID:PMC223838
Record Number:CaltechAUTHORS:GOUpnas67
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:GOUpnas67
Alternative URL:http://www.pnas.org/cgi/reprint/58/6/2321
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:8973
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:11 Oct 2007
Last Modified:16 Dec 2015 18:15

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