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Label-free photoacoustic microscopy of cytochrome c in cells

Zhang, Chi and Zhang, Yu and Yao, Da-Kang and Xia, Younan and Wang, Lihong V. (2012) Label-free photoacoustic microscopy of cytochrome c in cells. In: Photons Plus Ultrasound: Imaging and Sensing 2012. Proceedings of SPIE. No.8223. Society of Photo-optical Instrumentation Engineers (SPIE) , Bellingham, WA, Art. No. 82231W. ISBN 9780819488664. http://resolver.caltech.edu/CaltechAUTHORS:20180918-140710881

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Abstract

Cytochrome c is a heme protein normally bound to mitochondria and is important for mitochondrial electron transport and apoptosis initiation. Since cytochrome c is nonfluorescent, it is always labeled with fluorescent molecules for imaging, which, however, may affect normal cellular functions. Here, label-free photoacoustic microscopy (PAM) of mitochondrial cytochrome c was realized for the first time by utilizing the optical absorption around the Soret peak. PAM was demonstrated to be sensitive enough to image mitochondrial cytochrome c at 422 nm wavelength. Mitochondrial cytochrome c in the cytoplasm of fixed fibroblasts was clearly imaged by PAM as confirmed by fluorescent labeling. By showing mitochondrial cytochrome c in various cells, we demonstrated the feasibility of PAM for label-free histology of mouse ear sections. Therefore, PAM can sensitively image cytochrome c in unstained cells at 422 nm wavelength and has great potential for functional imaging of cytochrome c in live cells or in vivo.


Item Type:Book Section
Related URLs:
URLURL TypeDescription
https://doi.org/10.1117/12.906202DOIArticle
ORCID:
AuthorORCID
Wang, Lihong V.0000-0001-9783-4383
Additional Information:© 2012 Society of Photo-Optical Instrumentation Engineers (SPIE). This work was sponsored in part by National Institutes of Health grants R01 EB000712, R01 EB008085, R01 CA134539, U54 CA136398, R01 CA157277, and 5P60 DK02057933. L.W. has a financial interest in Microphotoacoustics, Inc. and Endra, Inc., which, however, did not support this work.
Funders:
Funding AgencyGrant Number
NIHR01 EB000712
NIHR01 EB008085
NIHR01 CA134539
NIHU54 CA136398
NIHR01 CA157277
NIH5P60 DK02057933
Subject Keywords:photoacoustic microscopy, cytochrome c, label-free
Record Number:CaltechAUTHORS:20180918-140710881
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20180918-140710881
Official Citation:Chi Zhang, Chi Zhang, Yu Zhang, Yu Zhang, Da-Kang Yao, Da-Kang Yao, Younan Xia, Younan Xia, Lihong V. Wang, Lihong V. Wang, } "Label-free photoacoustic microscopy of cytochrome c in cells", Proc. SPIE 8223, Photons Plus Ultrasound: Imaging and Sensing 2012, 82231W (23 February 2012); doi: 10.1117/12.906202; https://doi.org/10.1117/12.906202
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:89741
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:18 Sep 2018 23:18
Last Modified:18 Sep 2018 23:18

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