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Functional photoacoustic microscopy of pH

Chatni, M. Rameez and Yao, Junjie and Danielli, Amos and Favazza, Christopher P. and Maslov, Konstantin I. and Wang, Lihong V. (2012) Functional photoacoustic microscopy of pH. In: Photons Plus Ultrasound: Imaging and Sensing 2012. Proceedings of SPIE. No.8223. Society of Photo-optical Instrumentation Engineers (SPIE) , Bellingham, WA, Art. No. 82230N. ISBN 9780819488664.

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pH is a tightly regulated indicator of metabolic activity. In mammalian systems, imbalance of pH regulation may result from or result in serious illness. Even though the regulation system of pH is very robust, tissue pH can be altered in many diseases such as cancer, osteoporosis and diabetes mellitus. Traditional high-resolution optical imaging techniques, such as confocal microscopy, routinely image pH in cells and tissues using pH sensitive fluorescent dyes, which change their fluorescence properties with the surrounding pH. Since strong optical scattering in biological tissue blurs images at greater depths, high-resolution pH imaging is limited to penetration depths of 1mm. Here, we report photoacoustic microscopy (PAM) of commercially available pH-sensitive fluorescent dye in tissue phantoms. Using both opticalresolution photoacoustic microscopy (OR-PAM), and acoustic resolution photoacoustic microscopy (AR-PAM), we explored the possibility of recovering the pH values in tissue phantoms. In this paper, we demonstrate that PAM was capable of recovering pH values up to a depth of 2 mm, greater than possible with other forms of optical microscopy.

Item Type:Book Section
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URLURL TypeDescription ItemJournal Article
Wang, Lihong V.0000-0001-9783-4383
Additional Information:© 2012 Society of Photo-Optical Instrumentation Engineers (SPIE). This work was supported in part by National Institutes of Health grants U54 CA136398 and R01 EB000712. L. V. Wang has a financial interest in Microphotoacoustics, Inc., and Endra, Inc., which, however, did not support this work.
Funding AgencyGrant Number
NIHU54 CA136398
NIHR01 EB000712
Subject Keywords:Photoacoustic imaging, pH, optical imaging, quantitative photoacoustics, photoacoustic sensing
Series Name:Proceedings of SPIE
Issue or Number:8223
Record Number:CaltechAUTHORS:20180918-140711236
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Official Citation:M. Rameez Chatni, M. Rameez Chatni, Junjie Yao, Junjie Yao, Amos Danielli, Amos Danielli, Christopher P. Favazza, Christopher P. Favazza, Konstantin I. Maslov, Konstantin I. Maslov, Lihong V. Wang, Lihong V. Wang, } "Functional photoacoustic microscopy of pH", Proc. SPIE 8223, Photons Plus Ultrasound: Imaging and Sensing 2012, 82230N (23 February 2012); doi: 10.1117/12.907685;
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:89745
Deposited By: George Porter
Deposited On:18 Sep 2018 21:23
Last Modified:03 Oct 2019 20:18

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