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Optical-resolution photoacoustic microscopy of angiogenesis in a transgenic mouse model

Hu, Song and Oladipupo, Sunday and Yao, Junjie and Santeford, Andrea C. and Maslov, Konstantin and Kovalski, Joanna and Arbeit, Jeffrey M. and Wang, Lihong V. (2010) Optical-resolution photoacoustic microscopy of angiogenesis in a transgenic mouse model. In: Photons Plus Ultrasound: Imaging and Sensing 2010. Proceedings of SPIE. No.7564. Society of Photo-optical Instrumentation Engineers (SPIE) , Bellingham, WA, Art. No. 756406. ISBN 9780819479600.

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A major obstacle in studying angiogenesis is the inability to noninvasively image neovascular development in an individual animal. We applied optical-resolution photoacoustic microscopy (OR-PAM) to determine the kinetics of hypoxia-inducible factor-1 (HIF-1)-mediated angiogenesis in a transgenic mouse model. During continuous 30-day activation of HIF-1α, we used OR-PAM to monitor alterations in microvasculature in transgenic mice compared to nontransgenic mice. OR-PAM has demonstrated the potential to precisely monitor antiangiogenic therapy of human cancers, allowing for rapid determinations of therapeutic efficacy or resistance.

Item Type:Book Section
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Wang, Lihong V.0000-0001-9783-4383
Additional Information:© 2010 Society of Photo-Optical Instrumentation Engineers (SPIE). The authors thank Rebecca Sohn for mouse husbandry, Rick Bruick for the gift of the HIF-1α^(PPN) plasmid for construction of the TRE-HIF-1α^(PPN) transgenic mice, Adam Glick for the gift of the K5-rtTa transgenic mice, and ImClone for the generous gift of the DC101, and MF1 antibodies, Fulu Liu, Alyssa Gregory, Kyle Eash and Dan Link for help with bone marrow transplantation and advice on AMD3100 experiments. This work was supported by NCI R01-CA90722, National Institutes of Health Grants R01 EB000712, R01 NS46214, R01 EB008085, and U54 CA136398. L.V.W. has financial interest in Microphotoacoustics, Inc. and Endra, Inc., which, however, did not support this work.
Funding AgencyGrant Number
NIHR01 CA90722
NIHR01 EB000712
NIHR01 NS46214
NIHR01 EB008085
NIHU54 CA136398
Subject Keywords:Optical-resolution photoacoustic microscopy, chronic imaging, angiogenesis, HIF-1α
Record Number:CaltechAUTHORS:20180919-160039326
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Official Citation:Song Hu, Song Hu, Sunday Oladipupo, Sunday Oladipupo, Junjie Yao, Junjie Yao, Andrea C. Santeford, Andrea C. Santeford, Konstantin Maslov, Konstantin Maslov, Joanna Kovalski, Joanna Kovalski, Jeffrey M. Arbeit, Jeffrey M. Arbeit, Lihong V. Wang, Lihong V. Wang, } "Optical-resolution photoacoustic microscopy of angiogenesis in a transgenic mouse model", Proc. SPIE 7564, Photons Plus Ultrasound: Imaging and Sensing 2010, 756406 (23 February 2010); doi: 10.1117/12.841177;
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:89766
Deposited By: George Porter
Deposited On:20 Sep 2018 15:57
Last Modified:20 Sep 2018 15:57

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