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In vivo photoacoustic and ultrasonic mapping of rat sentinel lymph nodes with a modified commercial ultrasound imaging system

Erpelding, Todd N. and Kim, Chulhong and Pramanik, Manojit and Guo, Zijian and Dean, John and Jankovic, Ladislav and Maslov, Konstantin and Wang, Lihong V. (2010) In vivo photoacoustic and ultrasonic mapping of rat sentinel lymph nodes with a modified commercial ultrasound imaging system. In: Photons Plus Ultrasound: Imaging and Sensing 2010. Proceedings of SPIE. No.7564. Society of Photo-optical Instrumentation Engineers (SPIE) , Bellingham, WA, Art. No. 756418. ISBN 9780819479600.

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Sentinel lymph node biopsy (SLNB) has become the standard method for axillary staging in breast cancer patients, relying on invasive identification of sentinel lymph nodes (SLNs) following injection of blue dye and radioactive tracers. While SLNB achieves a low false negative rate (5-10%), it is an invasive procedure requiring ionizing radiation. As an alternative to SLNB, ultrasound-guided fine needle aspiration biopsy has been tested clinically. However, ultrasound alone is unable to accurately identify which lymph nodes are sentinel. Therefore, a non-ionizing and noninvasive detection method for accurate SLN mapping is needed. In this study, we successfully imaged methylene blue dye accumulation in vivo in rat axillary lymph nodes using a Phillips iU22 ultrasound imaging system adapted for photoacoustic imaging with an Nd:YAG pumped, tunable dye laser. Photoacoustic images of rat SLNs clearly identify methylene blue dye accumulation within minutes following intradermal dye injection and co-registered photoacoustic/ultrasound images illustrate lymph node position relative to surrounding anatomy. To investigate clinical translation, the imaging depth was extended up to 2.5 cm by adding chicken breast tissue on top of the rat skin surface. These results raise confidence that photoacoustic imaging can be used clinically for accurate, noninvasive SLN mapping.

Item Type:Book Section
Related URLs:
URLURL TypeDescription
Maslov, Konstantin0000-0003-3408-8840
Wang, Lihong V.0000-0001-9783-4383
Additional Information:© 2010 Society of Photo-Optical Instrumentation Engineers (SPIE). This research was supported by National Institutes of Health grants U54 CA136398 (Network for Translational Research), R01 EB000712, R01 NS46214, and R01 EB008085. We thank Victor Gornstein and Adam Schleicher for their contributions to modifying the Philips iU22 ultrasound imaging system. We thank Julie Margenthaler for helpful discussions on clinical breast cancer staging and treatment. LVW has a financial interest in Microphotoacoustics, Inc. and Endra, Inc., which, however, did not support this work. TNE, JD, and LJ are employees of Philips Research.
Funding AgencyGrant Number
NIHU54 CA136398
NIHR01 EB000712
NIHR01 NS46214
NIHR01 EB008085
Subject Keywords:Photoacoustic imaging, ultrasound imaging, sentinel lymph node, breast cancer
Series Name:Proceedings of SPIE
Issue or Number:7564
Record Number:CaltechAUTHORS:20180919-160039418
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Official Citation:Todd N. Erpelding, Todd N. Erpelding, Chulhong Kim, Chulhong Kim, Manojit Pramanik, Manojit Pramanik, Zijian Guo, Zijian Guo, John Dean, John Dean, Ladislav Jankovic, Ladislav Jankovic, Konstantin Maslov, Konstantin Maslov, Lihong V. Wang, Lihong V. Wang, } "In vivo photoacoustic and ultrasonic mapping of rat sentinel lymph nodes with a modified commercial ultrasound imaging system", Proc. SPIE 7564, Photons Plus Ultrasound: Imaging and Sensing 2010, 756418 (23 February 2010); doi: 10.1117/12.841858;
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:89767
Deposited By: George Porter
Deposited On:20 Sep 2018 16:17
Last Modified:03 Mar 2020 13:01

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