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Development of real-time photoacoustic microscopy

Wang, Lidai and Maslov, Konstantin and Yao, Junjie and Li, Li and Wang, Lihong V. (2011) Development of real-time photoacoustic microscopy. In: Photons Plus Ultrasound: Imaging and Sensing 2011. Proceedings of SPIE. No.7899. Society of Photo-optical Instrumentation Engineers (SPIE) , Bellingham, WA, Art. No. 78990R. ISBN 9780819484369. https://resolver.caltech.edu/CaltechAUTHORS:20180920-130933346

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Abstract

Photoacoustic tomography detecting ultrasound signals generated from photon absorption provides optical absorption contrast in vivo for structural, functional and molecular imaging. Although photoacoustic tomography technology has grown fast in recent years, real-time photoacoustic imaging with cellular spatial resolution are still strongly demanded. We developed a photoacoustic microscopy which has video-rate imaging capability with cellular spatial resolution. The system consists of a single-element focused ultrasound transducer, a fiber-based light-delivery subsystem, a voice-coil translation stage, a motion controller, and a data acquisition subsystem. A compact cube is employed to split optical and acoustic beams. The mass of the entire scanning photoacoustic probe is less than 40 grams, which minimizes potential vibrations and inertial effects, therefore, makes it capable to scan fast. The imaging system is capable of acquiring 20 cross-sectional (B-scan) images per second over 9 mm, and up to 40 B-scan images per second over 1 mm. Focused laser beams provide a lateral resolution of five microns. Confocal deployment of optical and acoustic focuses provides higher SNR than optical scanning approach. Micron-sized carbon particles flowing in silicone tubing and in vivo blood flows were imaged in video-rate, which demonstrated the capability to image highly dynamic biological processes in vivo with cellular resolution. This real-time high-resolution photoacoustic imaging system provides a promising approach for various in vivo imaging and quantitative studies.


Item Type:Book Section
Related URLs:
URLURL TypeDescription
https://doi.org/10.1117/12.875415DOIArticle
ORCID:
AuthorORCID
Wang, Lihong V.0000-0001-9783-4383
Additional Information:© 2011 Society of Photo-Optical Instrumentation Engineers (SPIE). We thank Song Hu, Dakang Yao, Liang Song, and Christopher Favazza for helpful discussions. This work was sponsored in part by National Institutes of Health grants R01 EB000712, R01 EB008085, R01 CA134539, U54 CA136398, R01 EB010049, and 5P60 DK02057933. L. V. Wang has a financial interest in Microphotoacoustics, Inc. and Endra, Inc., which, however, did not support this work.
Funders:
Funding AgencyGrant Number
NIHR01 EB000712
NIHR01 EB008085
NIHR01 CA134539
NIHU54 CA136398
NIHR01 EB010049
NIH5P60 DK02057933
Subject Keywords:Photoacoustic tomography, photoacoustic microscopy, fast scanning, real time, optical resolution, in vivo
Series Name:Proceedings of SPIE
Issue or Number:7899
Record Number:CaltechAUTHORS:20180920-130933346
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20180920-130933346
Official Citation:Lidai Wang, Lidai Wang, Konstantin Maslov, Konstantin Maslov, Junjie Yao, Junjie Yao, Li Li, Li Li, Lihong V. Wang, Lihong V. Wang, } "Development of real-time photoacoustic microscopy", Proc. SPIE 7899, Photons Plus Ultrasound: Imaging and Sensing 2011, 78990R (17 February 2011); doi: 10.1117/12.875415; https://doi.org/10.1117/12.875415
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:89790
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:21 Sep 2018 15:41
Last Modified:03 Oct 2019 20:19

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