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Photoacoustic image-guided needle biopsy of sentinel lymph nodes

Kim, Chulhong and Erpelding, Todd N. and Akers, Walter J. and Maslov, Konstantin and Song, Liang and Jankovic, Ladislav and Margenthaler, Julie A. and Achilefu, Samuel and Wang, Lihong V. (2011) Photoacoustic image-guided needle biopsy of sentinel lymph nodes. In: Photons Plus Ultrasound: Imaging and Sensing 2011. Proceedings of SPIE. No.7899. Society of Photo-optical Instrumentation Engineers (SPIE) , Bellingham, WA, Art. No. 78990K. ISBN 9780819484369.

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We have implemented a hand-held photoacoustic and ultrasound probe for image-guided needle biopsy using a modified clinical ultrasound array system. Pulsed laser light was delivered via bifurcated optical fiber bundles integrated with the hand-held ultrasound probe. We photoacoustically guided needle insertion into rat sentinel lymph nodes (SLNs) following accumulation of indocyanine green (ICG). Strong photoacoustic image contrast of the needle was achieved. After intradermal injection of ICG in the left forepaw, deeply positioned SLNs (beneath 2-cm thick chicken breast) were easily indentified in vivo and in real time. Further, we confirmed ICG uptake in axillary lymph nodes with in vivo and ex vivo fluorescence imaging. These results demonstrate the clinical potential of this hand-held photoacoustic system for facile identification and needle biopsy of SLNs for cancer staging and metastasis detection in humans.

Item Type:Book Section
Related URLs:
URLURL TypeDescription
Maslov, Konstantin0000-0003-3408-8840
Wang, Lihong V.0000-0001-9783-4383
Additional Information:© 2011 Society of Photo-Optical Instrumentation Engineers (SPIE). This work was supported in part by grants from National Institutes of Health (R01 EB000712, R01 EB008085, R01 NS46214, and U54 CA136398 -- the Network for Translational Research -- to L.V.W.). L.V.W. has a financial interest in Endra, Inc., which, however, did not support this work.
Funding AgencyGrant Number
NIHR01 EB000712
NIHR01 EB008085
NIHR01 NS46214
NIHU54 CA136398
Subject Keywords:Image-guided needle biopsy, photoacoustic imaging, sentinel lymph node biopsy
Series Name:Proceedings of SPIE
Issue or Number:7899
Record Number:CaltechAUTHORS:20180920-130933882
Persistent URL:
Official Citation:Chulhong Kim, Chulhong Kim, Todd N. Erpelding, Todd N. Erpelding, Walter J. Akers, Walter J. Akers, Konstantin Maslov, Konstantin Maslov, Liang Song, Liang Song, Ladislav Jankovic, Ladislav Jankovic, Julie A. Margenthaler, Julie A. Margenthaler, Samuel Achilefu, Samuel Achilefu, Lihong V. Wang, Lihong V. Wang, } "Photoacoustic image-guided needle biopsy of sentinel lymph nodes", Proc. SPIE 7899, Photons Plus Ultrasound: Imaging and Sensing 2011, 78990K (17 February 2011); doi: 10.1117/12.874956;
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:89795
Deposited By: George Porter
Deposited On:21 Sep 2018 14:34
Last Modified:03 Mar 2020 13:01

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