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Chronic label-free volumetric photoacoustic microscopy of melanoma cells in scaffolds in vitro

Cai, Xin and Zhang, Yu and Kim, Chulhong and Choi, Sung-Wook and Xia, Younan and Wang, Lihong V. (2011) Chronic label-free volumetric photoacoustic microscopy of melanoma cells in scaffolds in vitro. In: Photons Plus Ultrasound: Imaging and Sensing 2011. Proceedings of SPIE. No.7899. Society of Photo-optical Instrumentation Engineers (SPIE) , Bellingham, WA, Art. No. 789926. ISBN 9780819484369.

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Visualizing cells in three-dimensional (3D) scaffolds has been one of the major challenges in tissue engineering. Current imaging modalities have limitations. Microscopy, including confocal microscopy, cannot penetrate deeply (> 300 μm) into the scaffolds; X-ray micro-computed tomography (micro-CT) requires staining of the structure with a toxic agent such as osmium tetroxide. Here, we demonstrate photoacoustic microscopy (PAM) of the spatial distribution and temporal proliferation of melanoma cells inside three-dimensionally porous scaffolds with thicknesses over 1 mm. Melanoma cells have a strong intrinsic contrast which is easily imaged by label-free PAM with high sensitivity. Spatial distributions of the cells in the scaffold were well-resolved in PAM images. Moreover, we chronically imaged the same cell/scaffold constructs at different time points over 2 weeks. The number of cells in the scaffold was quantitatively measured from the PAM volumetric information. The cell proliferation profile obtained from PAM correlated well with that obtained using the traditional 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. We believe that PAM will become a useful imaging modality for tissue engineering applications, especially when thick scaffold constructs are involved, and that this modality can also be extended to image other cell types labeled with contrast agents.

Item Type:Book Section
Related URLs:
URLURL TypeDescription
Zhang, Yu0000-0001-8938-1927
Wang, Lihong V.0000-0001-9783-4383
Additional Information:© 2011 Society of Photo-Optical Instrumentation Engineers (SPIE). This work was supported in part by an NIH Director’s Pioneer Award (DP1 OD000798) and startup funds from Washington University in St. Louis (to X.Y.). This work was also sponsored by NIH grants (R01 EB000712, R01 NS46214, R01 EB008085, and U54 CA136398, to L.V.W.). Part of the work was performed at the Nano Research Facility (NRF), a member of the National Nanotechnology Infrastructure Network (NNIN), which is supported by the NSF under award ECS-0335765. L.V.W. has a financial interest in Microphotoacoustics, Inc. and Endra, Inc., which, however, did not support this work.
Funding AgencyGrant Number
NIHDP1 OD000798
NIHR01 EB000712
NIHR01 NS46214
NIHR01 EB008085
NIHU54 CA136398
Washington UniversityUNSPECIFIED
Subject Keywords:inverse opal scaffolds; photoacoustic microscopy; melanoma; tissue engineering; biomedical imaging
Series Name:Proceedings of SPIE
Issue or Number:7899
Record Number:CaltechAUTHORS:20180920-130934251
Persistent URL:
Official Citation:Xin Cai, Xin Cai, Yu Zhang, Yu Zhang, Chulhong Kim, Chulhong Kim, Sung-Wook Choi, Sung-Wook Choi, Younan Xia, Younan Xia, Lihong V. Wang, Lihong V. Wang, } "Chronic label-free volumetric photoacoustic microscopy of melanoma cells in scaffolds in vitro", Proc. SPIE 7899, Photons Plus Ultrasound: Imaging and Sensing 2011, 789926 (18 February 2011); doi: 10.1117/12.873226;
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:89799
Deposited By: George Porter
Deposited On:20 Sep 2018 22:15
Last Modified:03 Oct 2019 20:19

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