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High speed, inverted optical-resolution photoacoustic microscopy

Rao, Bin and Maslov, Konstantin and Danielli, Amos and Chen, Ruiming and Shung, K. Kirk and Zhou, Qifa and Wang, Lihong V. (2011) High speed, inverted optical-resolution photoacoustic microscopy. In: Photons Plus Ultrasound: Imaging and Sensing 2011. Proceedings of SPIE. No.7899. Society of Photo-optical Instrumentation Engineers (SPIE) , Bellingham, WA, Art. No. 78990T. ISBN 9780819484369. https://resolver.caltech.edu/CaltechAUTHORS:20180920-130934526

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Abstract

Photoacoustic microscopy (PAM) offers label-free, optical absorption contrast. A high-speed, high-resolution PAM system in an inverted microscope configuration with a laser pulse repetition rate of 100,000 Hz and a stationary ultrasonic transducer was built. Four-dimensional in vivo imaging of microcirculation in mouse skin was achieved at 18 three-dimensional volumes per second with repeated two-dimensional raster scans of 100 by 50 points. The corresponding twodimensional B-scan (50 A-lines) frame rate was 1800 Hz, and the one-dimensional A-scan rate was 90,000 Hz. The lateral resolution is 0.23±0.03 μm for Au nano-wire imaging, which is 2.0 times below the diffraction limit.


Item Type:Book Section
Related URLs:
URLURL TypeDescription
https://doi.org/10.1117/12.873568DOIArticle
ORCID:
AuthorORCID
Rao, Bin0000-0001-6494-3110
Chen, Ruiming0000-0002-5338-359X
Wang, Lihong V.0000-0001-9783-4383
Alternate Title:High speed inverted optical-resolution photoacoustic microscopy
Additional Information:© 2011 Society of Photo-Optical Instrumentation Engineers (SPIE). We gratefully thank the National Institutes of Health for supporting grants R01 EB000712, R01 EB008085, R01 CA134539, U54 CA136398, R01 CA157277, P41-EB2182. L.W. has a financial interest in Microphotoacoustics, Inc. and Endra, Inc., which, however, did not support this work.
Funders:
Funding AgencyGrant Number
NIHR01 EB000712
NIHR01 EB008085
NIHR01 CA134539
NIHU54 CA136398
NIHR01 CA157277
NIHP41-EB2182
Series Name:Proceedings of SPIE
Issue or Number:7899
Record Number:CaltechAUTHORS:20180920-130934526
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20180920-130934526
Official Citation:Bin Rao, Bin Rao, Konstantin Maslov, Konstantin Maslov, Amos Danielli, Amos Danielli, Ruiming Chen, Ruiming Chen, K. Kirk Shung, K. Kirk Shung, Qifa Zhou, Qifa Zhou, Lihong V. Wang, Lihong V. Wang, } "High speed inverted optical-resolution photoacoustic microscopy", Proc. SPIE 7899, Photons Plus Ultrasound: Imaging and Sensing 2011, 78990T (18 February 2011); doi: 10.1117/12.873568; https://doi.org/10.1117/12.873568
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:89802
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:20 Sep 2018 22:38
Last Modified:03 Oct 2019 20:19

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