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Dual-mode photoacoustic microscopy of carbon nanotube incorporated scaffolds in blood and biological tissues

Cai, Xin and Hu, Song and Paratala, Bhavna and Sitharaman, Balaji and Wang, Lihong V. (2011) Dual-mode photoacoustic microscopy of carbon nanotube incorporated scaffolds in blood and biological tissues. In: Photons Plus Ultrasound: Imaging and Sensing 2011. Proceedings of SPIE. No.7899. Society of Photo-optical Instrumentation Engineers (SPIE) , Bellingham, WA, Art. No. 78992I. ISBN 9780819484369.

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Three-dimensional scaffolds provide physical support and an adjustable microenvironment to facilitate vascularization of ischemic tissues; however, in vivo imaging of scaffold functioning is still challenging. Micro-CT, the current frequentlyused imaging modality for scaffold characterization, provides poor contrast for wet scaffold, which limits its in vivo applications. In this paper, dual modes of photoacoustic microscopy (PAM), using acoustic resolution PAM (AR-PAM) and optical resolution PAM (OR-PAM), were employed for imaging scaffolds in blood as well as in chicken breast tissues. By choosing different wavelengths, 570 nm and 638 nm, we spectroscopically differentiated the photoacoustic signals generated from blood and from carbon nanotube incorporated scaffolds. The ex vivo experiments demonstrated a lateral resolution of 45 μm and a maximum penetration of ~2 mm for AR-PAM, and a lateral resolution of 3 μm and a maximum penetration of ~660 μm for OR-PAM. OR-PAM further quantified the average pore size of scaffolds to be 100-200 μm in diameter. Our results suggest that PAM is a promising tool for in vivo monitoring of scaffold-induced angiogenesis as well as the degradability of scaffolds themselves.

Item Type:Book Section
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URLURL TypeDescription
Wang, Lihong V.0000-0001-9783-4383
Additional Information:© 2011 Society of Photo-Optical Instrumentation Engineers (SPIE). This work was sponsored by National Institutes of Health grants No. 1DP2OD007394-01(to B.S.), No. R01 EB000712, No. R01 EB008085, No. R01 CA134539, and No. U54 CA136398 (Network for Translational Research) (to L.V.W.). L.V.W. has a financial interest in Microphotoacoustics, Inc. and Endra, Inc., which, however, did not support this work.
Funding AgencyGrant Number
NIHR01 EB000712
NIHR01 EB008085
NIHR01 CA134539
NIHU54 CA136398
Subject Keywords:Three-dimensional scaffold; photoacoustic microscopy; carbon nanotube
Series Name:Proceedings of SPIE
Issue or Number:7899
Record Number:CaltechAUTHORS:20180920-130934742
Persistent URL:
Official Citation:Xin Cai, Xin Cai, Song Hu, Song Hu, Bhavna Paratala, Bhavna Paratala, Balaji Sitharaman, Balaji Sitharaman, Lihong V. Wang, Lihong V. Wang, } "Dual-mode photoacoustic microscopy of carbon nanotube incorporated scaffolds in blood and biological tissues", Proc. SPIE 7899, Photons Plus Ultrasound: Imaging and Sensing 2011, 78992I (18 February 2011); doi: 10.1117/12.873230;
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:89804
Deposited By: George Porter
Deposited On:20 Sep 2018 22:41
Last Modified:03 Oct 2019 20:19

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