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Effects of calibration factors and intensity dependent non-linearity on functional photoacoustic microscopy

Danielli, Amos and Yao, Junjie and Krumholz, Arie and Wang, Lihong V. (2011) Effects of calibration factors and intensity dependent non-linearity on functional photoacoustic microscopy. In: Photons Plus Ultrasound: Imaging and Sensing 2011. Proceedings of SPIE. No.7899. Society of Photo-optical Instrumentation Engineers (SPIE) , Bellingham, WA, Art. No. 78993C. ISBN 9780819484369.

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Functional photoacoustic microscopy is a valuable tool in quantifying hemoglobin oxygenation within single vessels. In several functional studies with this tool, quantitative sO_2 measurements were taken both in vitro and in vivo. Although in vitro measurements of sO_2 showed high agreement with expected values from premade samples, in practice, in vivo measurements were less accurate. The reported values of 70%-100% sO_2 in the arteries present large deviations from the expected range of 95-100%. Several factors, such as fluence wavelength dependence, optical wavelength range, and transducer central frequency have been suggested and investigated in order to understand these discrepancies. Despite additional knowledge of systematic errors arising from such factors, measuring the absolute value of sO_2 in vivo remains a challenge. All previous studies assumed linear dependence of the photoacoustic signal on absorption and used the linear least squares model. However, several factors, such as wavelength calibration errors, photodiode-wavelength dependence, and intensity dependent non-linearity, all of which may have a significant effect on the final calculation, have not been investigated. Here we evaluate both in vitro and in vivo the effects on sO_2 measurements of photodiode wavelength dependence, laser wavelength accuracy, and intensity dependent absorption of oxygenated and deoxygenated hemoglobin. We show that these factors may contribute significantly to the deviations in sO_2 calculations in vivo.

Item Type:Book Section
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Wang, Lihong V.0000-0001-9783-4383
Additional Information:© 2011 Society of Photo-Optical Instrumentation Engineers (SPIE).
Subject Keywords:Photoacoustic microscopy; non linear photoacoustic microscopy; oxygen saturation; optical saturation; photoacoustic tomography; photodiode calibration; wavelength calibration; OR-PAM
Series Name:Proceedings of SPIE
Issue or Number:7899
Record Number:CaltechAUTHORS:20180920-130934928
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Official Citation:Amos Danielli, Amos Danielli, Junjie Yao, Junjie Yao, Arie Krumholz, Arie Krumholz, Lihong V. Wang, Lihong V. Wang, } "Effects of calibration factors and intensity dependent non-linearity on functional photoacoustic microscopy", Proc. SPIE 7899, Photons Plus Ultrasound: Imaging and Sensing 2011, 78993C (28 February 2011); doi: 10.1117/12.875287;
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:89806
Deposited By: George Porter
Deposited On:20 Sep 2018 20:34
Last Modified:03 Oct 2019 20:19

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