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In-vivo imaging of nanoshell extravasation from solid tumor vasculature by photoacoustic microscopy

Li, Meng-Lin and Schwartz, Jon A. and Wang, James and Stoica, George and Wang, Lihong V. (2007) In-vivo imaging of nanoshell extravasation from solid tumor vasculature by photoacoustic microscopy. In: Photons Plus Ultrasound: Imaging and Sensing 2007: The Eighth Conference on Biomedical Thermoacoustics, Optoacoustics, and Acousto-optics. Proceedings of SPIE. No.6437. Society of Photo-optical Instrumentation Engineers (SPIE) , Bellingham, WA, Art. No. 64370B. ISBN 9780819465504. https://resolver.caltech.edu/CaltechAUTHORS:20180921-100903284

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Abstract

In this study, high resolution reflection-mode (backward-mode) photoacoustic microscopy (PAM) is used to noninvasively image progressive extravasation and accumulation of nanoshells within a solid tumor in vivo. This study takes advantage of the strong near-infrared absorption of nanoshells, a novel type of optically tunable gold nanoparticles that tend to extravasate from leaky tumor vasculatures (i.e., passive targeting) via the "enhanced permeability and retention" effect due to their nanoscale size. Tumors were grown in immunocompetent BALB/c mice by subcutaneous inoculation of CT26.wt murine colon carcinoma cells. PEGylated nanoshells with a peak optical absorption at ~800 nm were intravenously administered. Pre-scans prior to nanoshell injection were taken using a 584-nm laser source to highlight blood content and an 800-nm laser source to mark the background limit for nanoshell accumulation. After injection, the three-dimensional nanoshell distribution inside the tumor was monitored by PAM for 7 hours. Experimental results show that nanoshell accumulation is heterogeneous in tumors: more concentrated within the tumor cortex and largely absent from the tumor core. This correlates with others' observation that drug delivery within tumor cores is ineffective because of both high interstitial pressure and tendency to necrosis of tumor cores. Since nanoshells have been recently applied to thermal therapy for subcutaneous tumors, we anticipate that PAM will be important to this therapeutic technique.


Item Type:Book Section
Related URLs:
URLURL TypeDescription
https://doi.org/10.1117/12.697722DOIArticle
ORCID:
AuthorORCID
Stoica, George0000-0001-8071-4828
Wang, Lihong V.0000-0001-9783-4383
Additional Information:© 2007 Society of Photo-Optical Instrumentation Engineers (SPIE). We are grateful to Gina Lungu, Ovidiu Cracium, Kelly L. Gill for assistance with cell culture and veterinary procedure, and to Jung-Taek Oh, Hao F. Zhang, and Konstantin Maslov for technical advice and prior system development. This project is sponsored in part by the National Institutes of Health grants R01 EB000712 and R01 NS46214, and in part by the Advanced Technology Program at NIST, cooperative agreement #70NANB4H3040. Travel support from National Tsing Hua University, Taiwan is also greatly appreciated.
Funders:
Funding AgencyGrant Number
NIHR01 EB000712
NIHR01 NS46214
National Institute of Standards and Technology (NIST)70NANB4H3040
National Tsing Hua UniversityUNSPECIFIED
Subject Keywords:photoacoustic microscopy, nanoshell extravsation, enhanced permeability and retention effect
Series Name:Proceedings of SPIE
Issue or Number:6437
DOI:10.1117/12.697722
Record Number:CaltechAUTHORS:20180921-100903284
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20180921-100903284
Official Citation:Meng-Lin Li, Meng-Lin Li, Jon A. Schwartz, Jon A. Schwartz, James Wang, James Wang, George Stoica, George Stoica, Lihong V. Wang, Lihong V. Wang, } "In-vivo imaging of nanoshell extravasation from solid tumor vasculature by photoacoustic microscopy", Proc. SPIE 6437, Photons Plus Ultrasound: Imaging and Sensing 2007: The Eighth Conference on Biomedical Thermoacoustics, Optoacoustics, and Acousto-optics, 64370B (28 February 2007); doi: 10.1117/12.697722; https://doi.org/10.1117/12.697722
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:89843
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:24 Sep 2018 15:21
Last Modified:16 Nov 2021 00:38

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