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Quantitative photoacoustic imaging: correcting for heterogeneous light fluence distributions using diffuse optical tomography

Bauer, Adam Q. and Nothdurft, Ralph E. and Erpelding, Todd N. and Wang, Lihong V. and Culver, Joseph P. (2011) Quantitative photoacoustic imaging: correcting for heterogeneous light fluence distributions using diffuse optical tomography. Journal of Biomedical Optics, 16 (9). Art. No. 096016. ISSN 1083-3668. PMCID PMC3188642. https://resolver.caltech.edu/CaltechAUTHORS:20180921-105125387

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Abstract

The specificity of molecular and functional photoacoustic (PA) images depends on the accuracy of the photoacoustic absorption spectroscopy. The PA signal is proportional to the product of the optical absorption coefficient and local light fluence; quantitative PA measurements of the optical absorption coefficient therefore require an accurate estimation of optical fluence. Light-modeling aided by diffuse optical tomography (DOT) can be used to map the required fluence and to reduce errors in traditional PA spectroscopic analysis. As a proof-of-concept, we designed a tissue-mimicking phantom to demonstrate how fluence-related artifacts in PA images can lead to misrepresentations of tissue properties. To correct for these inaccuracies, the internal fluence in the tissue phantom was estimated by using DOT to reconstruct spatial distributions of the absorption and reduced scattering coefficients of multiple targets within the phantom. The derived fluence map, which only consisted of low spatial frequency components, was used to correct PA images of the phantom. Once calibrated to a known absorber, this method reduced errors in estimated absorption coefficients from 33% to 6%. These results experimentally demonstrate that combining DOT with PA imaging can significantly reduce fluence-related errors in PA images, while producing quantitatively accurate, high-resolution images of the optical absorption coefficient.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1117/1.3626212DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3188642PubMed CentralArticle
ORCID:
AuthorORCID
Wang, Lihong V.0000-0001-9783-4383
Additional Information:© 2011 SPIE. Paper 11132 received Mar. 18, 2011; revised manuscript received Jul. 27, 2011; accepted for publication Jul. 29, 2011; published online Sep. 1, 2011. This work was supported in part by NIH Grant Nos. R01-EB008085 and U54 CA136398.
Funders:
Funding AgencyGrant Number
NIHR01-EB008085
NIHU54 CA136398
Subject Keywords:quantitative photoacoustic imaging; diffuse optical tomography; light modeling; multimodal imaging
Issue or Number:9
PubMed Central ID:PMC3188642
Record Number:CaltechAUTHORS:20180921-105125387
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20180921-105125387
Official Citation:Adam Q. Bauer, Adam Q. Bauer, Ralph E. Nothdurft, Ralph E. Nothdurft, Joseph P. Culver, Joseph P. Culver, Todd N. Erpelding, Todd N. Erpelding, Lihong V. Wang, Lihong V. Wang, } "Quantitative photoacoustic imaging: correcting for heterogeneous light fluence distributions using diffuse optical tomography," Journal of Biomedical Optics 16(9), 096016 (1 September 2011). https://doi.org/10.1117/1.3626212
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:89882
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:21 Sep 2018 18:16
Last Modified:03 Oct 2019 20:20

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