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Engineered Alkane‐Hydroxylating Cytochrome P450_(BM3) Exhibiting Nativelike Catalytic Properties

Fasan, Rudi and Chen, Mike M. and Crook, Nathan C. and Arnold, Frances H. (2007) Engineered Alkane‐Hydroxylating Cytochrome P450_(BM3) Exhibiting Nativelike Catalytic Properties. Angewandte Chemie International Edition, 46 (44). pp. 8414-8418. ISSN 1433-7851. doi:10.1002/anie.200702616. https://resolver.caltech.edu/CaltechAUTHORS:20180925-122611378

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Abstract

Divide, evolve, and conquer: A domain‐based strategy (see scheme) was used to engineer high catalytic and coupling efficiency for propane hydroxylation in a multidomain cytochrome P450 enzyme. The engineered enzymes exhibit high total activities in whole‐cell bioconversions of propane to propanol under mild conditions, using air as oxidant.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1002/anie.200702616DOIArticle
ORCID:
AuthorORCID
Fasan, Rudi0000-0003-4636-9578
Crook, Nathan C.0000-0001-6165-1972
Arnold, Frances H.0000-0002-4027-364X
Alternate Title:Engineered Alkane‐Hydroxylating Cytochrome P450BM3 Exhibiting Nativelike Catalytic Properties
Additional Information:© 2007 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. Received: June 15, 2007; Published online: September 20, 2007. This work was supported by a Swiss National Science Foundation fellowship to R.F., a U.S. NSF Fellowship to M.M.C. and by the U.S. Army Research Office, ARO Contract DAAD19‐03‐D‐0004. We thank Dr. Christopher Snow for providing the homology model of P450_(BM3) reductase and Dr. Matthew W. Peters, Dr. Peter Meihnold, and Dr. Marco Landwehr for helpful discussions regarding the biotransformations and for providing access to DasGip fermenter.
Funders:
Funding AgencyGrant Number
Swiss National Science Foundation (SNSF)UNSPECIFIED
NSFUNSPECIFIED
Army Research Office (ARO)DAAD19‐03‐D‐0004
Subject Keywords:alkane oxidation; biotransformations; cytochrome P450; directed evolution; protein engineering
Issue or Number:44
DOI:10.1002/anie.200702616
Record Number:CaltechAUTHORS:20180925-122611378
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20180925-122611378
Official Citation:Fasan, R. , Chen, M. , Crook, N. and Arnold, F. (2007), Engineered Alkane‐Hydroxylating Cytochrome P450BM3 Exhibiting Nativelike Catalytic Properties. Angewandte Chemie International Edition, 46: 8414-8418. doi:10.1002/anie.200702616
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:89917
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:25 Sep 2018 21:53
Last Modified:16 Nov 2021 00:39

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