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Deep penetrating photoacoustic tomography in biological tissues

Ku, Geng and Wang, Xueding and Xie, Xueyi and Stoica, George and Wang, Lihong V. (2005) Deep penetrating photoacoustic tomography in biological tissues. In: Photons Plus Ultrasound: Imaging and Sensing 2005: The Sixth Conference on Biomedical Thermoacoustics, Optoacoustics, and Acousto-optics. Proceedings of SPIE. No.5697. Society of Photo-optical Instrumentation Engineers (SPIE) , Bellingham, WA, pp. 117-126. ISBN 9780819456717.

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Photoacoustic tomography (PAT) in a circular scanning configuration was developed to image the deeply embedded optical heterogeneity in biological tissues. Based on the intrinsic contrast between blood and chicken breast muscle, an embedded blood object that was 5 cm deep in the tissue was detected using pulsed laser light at a wavelength of 1064 nm. Compared with detectors for flat active surfaces, cylindrically focused ultrasonic transducers can reduce the interference generated from the off-plane photoacoustic sources and make the image in the scanning plane clearer. While the optical penetration was optimized with near-infrared laser pulses of 800 nm in wavelength, the optical contrast was enhanced by indocyanine green (ICG) whose absorption peak matched the laser wavelength. This optimized PAT was able to image fine objects embedded at a depth of up to 5.2-cm, which is 6.2 times the 1/e optical penetration depth, in chicken breast muscle, at a resolution of < ~750 microns with a sensitivity of <7 pmol of ICG in blood. The resolution was found to deteriorate slowly with increasing imaging depth.

Item Type:Book Section
Related URLs:
URLURL TypeDescription
Stoica, George0000-0001-8071-4828
Wang, Lihong V.0000-0001-9783-4383
Additional Information:© 2005 Society of Photo-Optical Instrumentation Engineers (SPIE). We thank M. Sivaramakrishnan and K. Song for lab assistance. This study is sponsored in part by the National Institute of Health grants R01 EB000712 and R01 NS46214 and the Texas ARP grant 000512-0063-2001.
Funding AgencyGrant Number
NIHR01 EB000712
NIHR01 NS46214
Texas Higher Education Coordinating Board000512-0063-2001
Subject Keywords:photoacoustic tomography, medical imaging, biological tissue, breast imaging, spatial resolution, image reconstruction, optical contrast agent
Series Name:Proceedings of SPIE
Issue or Number:5697
Record Number:CaltechAUTHORS:20180926-080928662
Persistent URL:
Official Citation:Geng Ku, Geng Ku, Xueding Wang, Xueding Wang, Xueyi Xie, Xueyi Xie, George Stoica, George Stoica, Lihong V. Wang, Lihong V. Wang, } "Deep penetrating photoacoustic tomography in biological tissues", Proc. SPIE 5697, Photons Plus Ultrasound: Imaging and Sensing 2005: The Sixth Conference on Biomedical Thermoacoustics, Optoacoustics, and Acousto-optics, (25 April 2005); doi: 10.1117/12.588798;
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:89944
Deposited By: Tony Diaz
Deposited On:26 Sep 2018 18:22
Last Modified:16 Nov 2021 00:39

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