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Evolved orthogonal ribosomes

Chin, Jason W. and Wang, Kaihang and Neumann, Heinz (2013) Evolved orthogonal ribosomes. Patent number: US8497231B2.

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There is provided a method for evolving an orthogonal rRNA molecule, comprising the steps of: providing one or more libraries of mutant orthogonal rRNA molecules and introducing the libraries into cells such that the orthogonal rRNA is incorporated into ribosomes to provide orthogonal ribosomes; providing one or more orthogonal mRNA molecules which (i) are not translated by natural ribosomes, and (ii) comprise one or more orthogonal mRNA codons; assaying the translation of the orthogonal mRNA and selecting the orthogonal rRNA molecules which translate the orthogonal mRNA, wherein the assay in step (c) requires translation of one or more orthogonal mRNA codons in the orthogonal mRNA; and orthogonal ribosomes incorporating such rRNA molecules.

Item Type:Patent
Related Patents:US 2010/0105565 A1
Patent Classification:C12N15/11 C12N15/111 C12P21/02 C40B10/00 C40B40/08 C12N2320/13
Patent Assignee:Medical Research Council, London
Related URLs:
URLURL TypeDescription Patents
Chin, Jason W.0000-0003-1219-4757
Wang, Kaihang0000-0001-7657-8755
Neumann, Heinz0000-0001-9657-1913
Additional Information:Application filed:2007-11-28. Application published: 2010-04-29. Application granted: 2013-07-30. Patent published:2013-07-30. Patent status: Expired - Fee Related. Adjusted expiration: 2028-03-16. Patent Published as: CA2670588A1;EP2099912A2;JP2010510798A;JP5307020B2;US2010105565A1;US8497231B2;WO2008065398A2;WO2008065398A3
Record Number:CaltechAUTHORS:20180926-093116068
Persistent URL:
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:89951
Deposited By: Donna Wrublewski
Deposited On:09 Mar 2020 14:56
Last Modified:12 Oct 2022 16:57

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