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Generation of tissue-specific transgenic birds with lentiviral vectors

Scott, Benjamin B. and Lois, Carlos (2005) Generation of tissue-specific transgenic birds with lentiviral vectors. Proceedings of the National Academy of Sciences of the United States of America, 102 (45). pp. 16443-16447. ISSN 0027-8424. PMCID PMC1275601. doi:10.1073/pnas.0508437102.

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Birds are of great interest for a variety of research purposes, and effective methods for manipulating the avian genome would greatly accelerate progress in fields that rely on birds as model systems for biological research, such as developmental biology and behavioral neurobiology. Here, we describe a simple and effective method for producing transgenic birds. We used lentiviral vectors to produce transgenic quails that express GFP driven by the human synapsin gene I promoter. Expression of GFP was specific to neurons and consistent across multiple generations. Expression was sufficient to allow visualization of individual axons and dendrites of neurons in vivo by intrinsic GFP fluorescence. Tissue-specific transgene expression at high levels provides a powerful tool for biological research and opens new avenues for genetic manipulation in birds.

Item Type:Article
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URLURL TypeDescription CentralArticle
Lois, Carlos0000-0002-7305-2317
Additional Information:© 2005 The National Academy of Sciences. Freely available online through the PNAS open access option. Communicated by Susumu Tonegawa, Massachusetts Institute of Technology, Cambridge, MA, September 27, 2005 (received for review January 12, 2005). B.B.S. thanks A. Stolfi for assistance with egg injections and H. Y. Chung and L. Smith for help with figures. Author contributions: B.B.S. and C.L. designed research; B.B.S. performed research; B.B.S. analyzed data; and B.B.S. wrote the paper. Conflict of interest statement: No conflicts declared.
Subject Keywords:avian; genetics; lentivirus; synapsin; neuron
Issue or Number:45
PubMed Central ID:PMC1275601
Record Number:CaltechAUTHORS:20180926-141338632
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Official Citation:Generation of tissue-specific transgenic birds with lentiviral vectors. Benjamin B. Scott, Carlos Lois. Proceedings of the National Academy of Sciences Nov 2005, 102 (45) 16443-16447; DOI: 10.1073/pnas.0508437102
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:89970
Deposited By: Tony Diaz
Deposited On:26 Sep 2018 21:41
Last Modified:16 Nov 2021 00:39

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