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Distribution of extracellular flavins in a coastal marine basin and their relationship to redox gradients and microbial community members

Monteverde, Danielle R. and Sylvan, Jason B. and Suffridge, Christopher and Baronas, J. Jotautas and Fichot, Erin and Fuhrman, Jed and Berelson, William and Sanudo-Wilhelmy, Sergio A. (2018) Distribution of extracellular flavins in a coastal marine basin and their relationship to redox gradients and microbial community members. Environmental Science and Technology, 52 (21). pp. 12265-12274. ISSN 0013-936X. https://resolver.caltech.edu/CaltechAUTHORS:20180927-111747764

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Abstract

The flavins (including flavin mononucleotide (FMN) and riboflavin (RF)) are a class of organic compounds synthesized by organisms to assist in critical redox reactions. While known to be secreted extracellularly by some species in laboratory-based cultures, flavin concentrations are largely unreported in the natural environment. Here, we present pore water and water column profiles of extracellular flavins (FMN and RF) and two degradation products (lumiflavin and lumichrome) from a coastal marine basin in the Southern California Bight alongside ancillary geochemical and 16S rRNA microbial community data. Flavins were detectable at picomolar concentrations in the water column (93–300 pM FMN, 14–40 pM RF) and low nanomolar concentrations in pore waters (250–2070 pM FMN, 11–210 pM RF). Elevated pore water flavin concentrations displayed an increasing trend with sediment depth and were significantly correlated with the total dissolved Fe (negative) and Mn (positive) concentrations. Network analysis revealed a positive relationship between flavins and the relative abundance of Dehalococcoidia and the MSBL9 clade of Planctomycetes, indicating possible secretion by members of these lineages. These results suggest that flavins are a common component of the so-called shared extracellular metabolite pool, especially in anoxic marine sediments where they exist at physiologically relevant concentrations for metal oxide reduction.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1021/acs.est.8b02822DOIArticle
https://pubs.acs.org/doi/suppl/10.1021/acs.est.8b02822PublisherSupporting Information
ORCID:
AuthorORCID
Monteverde, Danielle R.0000-0002-0198-8220
Sylvan, Jason B.0000-0001-7361-7472
Suffridge, Christopher0000-0001-8466-6368
Baronas, J. Jotautas0000-0002-4027-3965
Additional Information:© 2018 American Chemical Society. Received: May 28, 2018; Revised: September 24, 2018; Accepted: September 27, 2018; Published: September 27, 2018. We thank Nick Rollins, Elias Karkabi, and Lynda Cutter (University of Southern California) for help in sample collection and analysis, Willie Haskell and Doug Hammond (USC) for cruise support, Cole Hastings for help with cell counts, and Annette Rowe (USC) for flavin discussions. Support for this work was provided by National Science Foundation Grants OCE 1435666 and 1260692, startup funds from Texas A&M University to JBS, and Caltech Division of Geological and Planetary Sciences Geobiology Postdoc salary for DRM. The authors declare no competing financial interest.
Funders:
Funding AgencyGrant Number
NSFOCE-1435666
NSFOCE-1260692
Texas A&M UniversityUNSPECIFIED
Caltech Division of Geological and Planetary SciencesUNSPECIFIED
Issue or Number:21
Record Number:CaltechAUTHORS:20180927-111747764
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20180927-111747764
Official Citation:Distribution of Extracellular Flavins in a Coastal Marine Basin and Their Relationship to Redox Gradients and Microbial Community Members. Danielle R. Monteverde, Jason B. Sylvan, Christopher Suffridge, J. Jotautas Baronas, Erin Fichot, Jed Fuhrman, William Berelson, and Sergio A. Sañudo-Wilhelmy. Environmental Science & Technology 2018 52 (21), 12265-12274 DOI: 10.1021/acs.est.8b02822
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:89990
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:27 Sep 2018 18:25
Last Modified:03 Oct 2019 20:21

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