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Maintaining trunk neural crest cells as crestospheres

Mohlin, Sofie and Kunttas, Ezgi and Persson, Camilla U. and Abdel-Haq, Reem and Castillo, Aldo and Murko, Christina and Bronner, Marianne E. and Kerosuo, Laura (2018) Maintaining trunk neural crest cells as crestospheres. . (Unpublished)

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Neural crest cells have broad migratory and differentiative ability that differs according to their axial level of origin. However, their transient nature has limited understanding of their stem cell and self-renewal properties. While an in vitro culture method has made it possible to maintain cranial neural crest cells as self-renewing multipotent crestospheres (Kerosuo et al., 2015), these same conditions failed to preserve trunk neural crest in a stem-like state. Here we optimize culture conditions for maintenance of trunk crestospheres, comprised of both neural crest stem and progenitor cells. Trunk crestospheres display elevated expression of neural crest cell markers as compared to those characteristic of neural tube or mesodermal fates. Moreover, trunk crestospheres have increased expression of trunk-related markers as compared to cranial genes. Finally, we use lentiviral transduction as a tool to manipulate gene expression in trunk crestospheres. Taken together, this method enables long-term in vitro maintenance and manipulation of trunk neural crest cells in a premigratory stem or early progenitor state to probe the mechanisms underlying their stemness and lineage decisions.

Item Type:Report or Paper (Discussion Paper)
Related URLs:
URLURL TypeDescription Paper
Mohlin, Sofie0000-0002-2458-3963
Abdel-Haq, Reem0000-0002-7418-5736
Murko, Christina0000-0003-2281-0031
Bronner, Marianne E.0000-0003-4274-1862
Kerosuo, Laura0000-0001-6710-3512
Additional Information:The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. This article is a US Government work. It is not subject to copyright under 17 USC 105 and is also made available for use under a CC0 license. This work was supported by the Swedish Childhood Cancer Foundation (to SM), the Mary Bevé Foundation (to SM), Magnus Bergvall’s Foundation (to SM), the Thelma Zoéga Foundation (to SM), Hans von Kantzow Foundation (to SM), the Royal Physiographic Society of Lund (to SM), NIH Ruth L. Kirschstein NRSA F32HD087026 (to EK), NIH R01DE024157 (to MB), the Academy of Finland (to LK), Sigrid Juselius Foundation (to LK), and in part by the Division of Intramural Research of the National Institute of Dental and Craniofacial Research at the National Institutes of Health, Department of Health and Human Services.
Funding AgencyGrant Number
Swedish Childhood Cancer FoundationUNSPECIFIED
Mary Bevé FoundationUNSPECIFIED
Magnus Bergvall’s FoundationUNSPECIFIED
Thelma Zoéga FoundationUNSPECIFIED
Hans von Kantzow FoundationUNSPECIFIED
Royal Physiographic Society of LundUNSPECIFIED
NIH Postdoctoral FellowshipF32HD087026
Academy of FinlandUNSPECIFIED
Sigrid Juselius FoundationUNSPECIFIED
National Institute of Dental and Craniofacial Research (NIDCR)UNSPECIFIED
Subject Keywords:crestospheres; neural crest stem cells; trunk neural crest; cranial neural crest; chick embryo; Gallus gallus; cell cultures, stem cell maintenance
Record Number:CaltechAUTHORS:20180927-114223449
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:89997
Deposited By: George Porter
Deposited On:28 Sep 2018 15:05
Last Modified:25 Mar 2019 18:26

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