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Draxin alters laminin expression during basement membrane reorganization to control cranial neural crest EMT

Hutchins, Erica J. and Bronner, Marianne E. (2018) Draxin alters laminin expression during basement membrane reorganization to control cranial neural crest EMT. . (Unpublished) http://resolver.caltech.edu/CaltechAUTHORS:20180927-114224386

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Abstract

Premigratory neural crest cells arise within the dorsal neural tube and subsequently undergo an epithelial-to-mesenchymal transition (EMT) to leave the neuroepithelium and initiate migration. Draxin is a Wnt modulator that has been shown to control the timing of cranial neural crest EMT. Here we show that this process is accompanied by three stages of remodeling of the basement membrane protein laminin, from regression to expansion and channel formation. Loss of Draxin results in blocking laminin remodeling at the regression stage, whereas ectopic maintenance of Draxin blocks remodeling at the expansion stage. The latter effect is rescued by addition of Snail2, previously shown to be downstream of Draxin. Our results demonstrate an essential function for the Wnt modulator Draxin in regulating basement membrane remodeling during cranial neural crest EMT.


Item Type:Report or Paper (Discussion Paper)
Related URLs:
URLURL TypeDescription
https://doi.org/10.1101/407882DOIDiscussion Paper
ORCID:
AuthorORCID
Hutchins, Erica J.0000-0002-4316-0333
Bronner, Marianne E.0000-0003-4274-1862
Additional Information:The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission. We thank Dr. M. Simoes-Costa for providing the NC1.1 M3:EGFP construct, and Dr. M. Cheung for providing the pCIG-V5-Snail2-IRES-nls-GFP construct (Addgene plasmid # 44282). This work was supported by a National Institutes of Health grant (R01DE024157 and PO1HD037105 to M.E. Bronner), and a Ruth L. Kirschstein National Research Service Award (F32DE026355 to E.J. Hutchins). The authors declare no competing interests.
Funders:
Funding AgencyGrant Number
NIHR01DE024157
NIHPO1HD037105
NIH Postdoctoral FellowshipF32DE026355
Subject Keywords:Neural crest; Laminin; Draxin; Snail2; Epithelial-to-Mesenchymal Transition
Record Number:CaltechAUTHORS:20180927-114224386
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20180927-114224386
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:90005
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:27 Sep 2018 22:02
Last Modified:25 Mar 2019 18:48

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