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Functional analysis of Scratch2 domains: implications in the evolution of Snail transcriptional repressors

Nakamura Kanno, Tatiane Yumi and Soares Fogo, Mariana and Purcell Goes, Carolina and Vieceli, Felipe Monteleone and Yan, Chao Yun Irene (2018) Functional analysis of Scratch2 domains: implications in the evolution of Snail transcriptional repressors. . (Unpublished) http://resolver.caltech.edu/CaltechAUTHORS:20180927-114224493

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Abstract

The Snail superfamily of transcription factors have a modular organization and their similarities and divergences are the basis for subdividing the superfamily into the Snail1/2 and Scratch families. As it is generally accepted that the Snail and Scratch families originated through gene duplication, understanding the functional contribution of each module could provide us with further insight about the molecular and functional evolution of the Snail superfamily. Thus, in this work, we investigated the function of the SNAG and SCRATCH domains in chicken Scratch2. Through evolutionary comparison analysis we identified a novel HINGE domain that lies between the SNAG and SCRATCH domain. Similar to members of the Snail1/2 families, Scratch2-mediated transcriptional repression requires SNAG and nuclear localization requires the zinc-finger domain. We also identified a novel HINGE domain that lies between the SNAG and SCRATCH domain. HINGE is highly conserved in amniotes. Single mutations of the conserved Tyrosine and Serine residues of HINGE downregulated Scratch2-mediated transcriptional repression. This effect depended on the presence of the SCRATCH domain.


Item Type:Report or Paper (Discussion Paper)
Related URLs:
URLURL TypeDescription
https://doi.org/10.1101/410761DOIDiscussion Paper
ORCID:
AuthorORCID
Purcell Goes, Carolina0000-0002-4086-3519
Vieceli, Felipe Monteleone0000-0001-5142-8224
Yan, Chao Yun Irene0000-0003-0527-5719
Additional Information:The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license. The authors thank Dr. Paulo Sérgio Lopes Oliveira for discussions about protein structure, Dr. Cristóvão Albuquerque for editing assistance and Dr. Ali Brivanlou for generously sharing lab space and reagents.
Subject Keywords:Scratch, Transcription factor, Chicken Embryo, Snail superfamily, DNA binding protein, Nuclear translocation, Protein domain
Record Number:CaltechAUTHORS:20180927-114224493
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20180927-114224493
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:90006
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:27 Sep 2018 22:00
Last Modified:27 Sep 2018 22:00

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