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Dissecting heterogeneous cell-populations across signaling and disease conditions with PopAlign

Chen, Sisi and Park, Jong H. and Charles, Emeric and Rivaud, Paul and Haliburton, John and Pichiorri, Flavia and Thomson, Matt (2018) Dissecting heterogeneous cell-populations across signaling and disease conditions with PopAlign. . (Unpublished)

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Single-cell measurement techniques can now probe gene expression in heterogeneous cell populations from the human body across a range of environmental and physiological conditions. However, new mathematical and computational methods are required to represent and analyze gene expression changes that occur in complex mixtures of single cells as they respond to signals, drugs, or disease states. Here, we introduce a mathematical modeling platform, PopAlign, that automatically identifies subpopulations of cells within a heterogeneous mixture, and tracks gene expression and cell abundance changes across subpopulations by constructing and comparing probabilistic models. We apply PopAlign to discover specific categories of signaling responses within primary human immune cells as well as patient-specific disease signatures in multiple myeloma that are obscured by techniques like tSNE. We anticipate that PopAlign will enable large scale studies of natural and engineered cell populations as they respond to drugs, signals or physiological change.

Item Type:Report or Paper (Discussion Paper)
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Additional Information:The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Record Number:CaltechAUTHORS:20180927-114224716
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:90008
Deposited By: George Porter
Deposited On:27 Sep 2018 21:04
Last Modified:13 Feb 2020 17:05

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