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Laser-induced photoacoustic tomography enhanced with an optical contrast agent

Wang, Xueding and Ku, Geng and Xie, Xueyi and Wegiel, Malgorzata A. and Bornhop, Darryl J. and Stoica, George and Wang, Lihong V. (2004) Laser-induced photoacoustic tomography enhanced with an optical contrast agent. In: Photons Plus Ultrasound: Imaging and Sensing. Proceedings of SPIE. No.5320. Society of Photo-optical Instrumentation Engineers (SPIE) , Bellingham, WA, pp. 77-82. ISBN 9780819452283.

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Optical contrast agents, such as indocyanine dyes, nano-particles and their functional derivatives, have been widely applied to enhance the sensitivity and specificity of optical imaging. However, due to the overwhelming scattering of light in biological tissues, the spatial resolution of traditional optical imaging degrades drastically as the imaging depth increases. For the first time to our knowledge, non-invasive in vivo photoacoustic imaging of an optical contrast agent, distributed in the rat brain, was implemented with near-infrared light. Injection of indocyanine green polyethylene glycol, a contrast agent with a high absorption at the 805-nm wavelength, into the circulatory system of a rat enhanced the absorption contrast between the blood vessels and the background brain tissues. Because near-infrared light can penetrate deep into the brain tissues through the skin and skull, we were able to successfully reconstruct the vascular distribution in the rat brain from the detected photoacoustic signals. The dynamic concentration of this contrast agent in the brain blood after the intravenous injection was also studied. This work proved that the distribution of an exogenous contrast agent in biological tissues can be imaged clearly and accurately by photoacoustic tomography. This new technology has high potential for application in dynamic and molecular medical imaging.

Item Type:Book Section
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Stoica, George0000-0001-8071-4828
Wang, Lihong V.0000-0001-9783-4383
Additional Information:© 2004 Society of Photo-Optical Instrumentation Engineers (SPIE).
Subject Keywords:photoacoustic tomography, contrast agent, indocyanine green, near-infrared light, neuroimaging
Series Name:Proceedings of SPIE
Issue or Number:5320
Record Number:CaltechAUTHORS:20180927-114226623
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Official Citation:Xueding Wang, Xueding Wang, Geng Ku, Geng Ku, Xueyi Xie, Xueyi Xie, Malgorzata A. Wegiel, Malgorzata A. Wegiel, Darryl J. Bornhop, Darryl J. Bornhop, George Stoica, George Stoica, Lihong V. Wang, Lihong V. Wang, } "Laser-induced photoacoustic tomography enhanced with an optical contrast agent", Proc. SPIE 5320, Photons Plus Ultrasound: Imaging and Sensing, (12 July 2004); doi: 10.1117/12.532434;
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:90027
Deposited By: George Porter
Deposited On:28 Sep 2018 16:14
Last Modified:16 Nov 2021 00:40

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