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Development of lung tissue phantoms for bioluminescent imaging

Kumar, D. and Cong, Wenxiang and Bohenkamp, Frank and Kakaday, Tarun and Taft, Peter and Wang, Lihong V. and McLennan, Geoffrey and Hoffman, Eric A. and Wang, Ge (2004) Development of lung tissue phantoms for bioluminescent imaging. In: Developments in X-Ray Tomography IV. Proceedings of SPIE. No.5535. Society of Photo-optical Instrumentation Engineers (SPIE) , Bellingham, WA, pp. 687-693. ISBN 9780819454737.

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White nylon material was chosen to make cylindrical tissue phantoms for development of bioluminescence tomography techniques. A low-level light source, delivered through an optic fiber of core diameter 200 μm, was placed at different locations on one phantom surface. The light travels through the phantom, reaches the external surface, and is captured by a liquid nitrogen-cooled CCD camera. The scattering, absorption, and anisotropy parameters of the phantom are obtained by matching the measured light transmission profiles to the profiles generated by the TracePro software. The perturbation analysis, with the homogeneous phantoms, demonstrated that the imaging system is sufficiently sensitive to capture intensity change of higher than 0.5nW/cm2 or a location shift of the light source of more than 200 microns. It is observed that the system can distinguish two point light sources with separation of about 2 mm. The perturbation analysis is also performed with the heterogeneous phantom. Based on our data, we conclude that there is inherent tomographic information in bioluminescent measures taken on the external surface of the mouse, which suggests the feasibility of bioluminescence tomography for biomedical research using the small animals, especially the mice.

Item Type:Book Section
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Wang, Lihong V.0000-0001-9783-4383
Additional Information:© 2004 Society of Photo-Optical Instrumentation Engineers (SPIE). This work is partially supported by the NIH/NIBIB grant 1R21EB001685.
Funding AgencyGrant Number
Subject Keywords:Bioluminescence tomography (BLT), tissue phantom, optic fiber, light transmission, perturbation analysis
Series Name:Proceedings of SPIE
Issue or Number:5535
Record Number:CaltechAUTHORS:20180927-114227556
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Official Citation:Durairaj Kumar, Durairaj Kumar, Wenxiang Cong, Wenxiang Cong, Frank Bohenkamp, Frank Bohenkamp, Tarun Kakaday, Tarun Kakaday, Peter Taft, Peter Taft, Lihong V. Wang, Lihong V. Wang, Geoffrey McLennan, Geoffrey McLennan, Eric A. Hoffman, Eric A. Hoffman, Ge Wang, Ge Wang, } "Development of lung tissue phantoms for bioluminescent imaging", Proc. SPIE 5535, Developments in X-Ray Tomography IV, (26 October 2004); doi: 10.1117/12.560530;
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:90037
Deposited By: George Porter
Deposited On:28 Sep 2018 23:01
Last Modified:16 Nov 2021 00:40

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