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Generation of transgenic birds with replication-deficient lentiviruses

Scott, Benjamin B. and Lois, Carlos (2006) Generation of transgenic birds with replication-deficient lentiviruses. Nature Protocols, 1 . pp. 1406-1411. ISSN 1754-2189. doi:10.1038/nprot.2006.187.

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Birds are of great interest as an animal model in biological research and for commercial applications as a bioreactor. Effective methods for manipulating the avian genome would accelerate progress in fields such as developmental biology and behavioral neurobiology, which traditionally have relied on birds as model systems for biological research. Here we describe a simple and effective protocol for producing transgenic birds using lentiviral vectors that can be used to achieve tissue-specific transgene expression at high levels. The time allotted for the procedure depends upon the species of bird; adult transgenic quails can be generated in approximately 5 months.

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Lois, Carlos0000-0002-7305-2317
Additional Information:© 2006 Springer Nature Limited. Published 02 November 2006. The authors declare no competing financial interests.
Record Number:CaltechAUTHORS:20180928-105832049
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:90051
Deposited By: Tony Diaz
Deposited On:28 Sep 2018 22:11
Last Modified:16 Nov 2021 00:40

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