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Distinct Mammalian Precursors Are Committed to Generate Neurons with Defined Dendritic Projection Patterns

Kelsch, Wolfgang and Mosley, Colleen P. and Lin, Chia-Wei and Lois, Carlos (2007) Distinct Mammalian Precursors Are Committed to Generate Neurons with Defined Dendritic Projection Patterns. PLoS Biology, 5 (11). Art. No. e300. ISSN 1544-9173. PMCID PMC2071944. https://resolver.caltech.edu/CaltechAUTHORS:20181001-113519846

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Abstract

The mechanisms that regulate how dendrites target different neurons to establish connections with specific cell types remain largely unknown. In particular, the formation of cell-type–specific connectivity during postnatal neurogenesis could be either determined by the local environment of the mature neuronal circuit or by cell-autonomous properties of the immature neurons, already determined by their precursors. Using retroviral fate mapping, we studied the lamina-specific dendritic targeting of one neuronal type as defined by its morphology and intrinsic somatic electrical properties in neonatal and adult neurogenesis. Fate mapping revealed the existence of two separate populations of neuronal precursors that gave rise to the same neuronal type with two distinct patterns of dendritic targeting—innervating either a deep or superficial lamina, where they connect to different types of principal neurons. Furthermore, heterochronic and heterotopic transplantation demonstrated that these precursors were largely restricted to generate neurons with a predetermined pattern of dendritic targeting that was independent of the host environment. Our results demonstrate that, at least in the neonatal and adult mammalian brain, the pattern of dendritic targeting of a given neuron is a cell-autonomous property of their precursors.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1371/journal.pbio.0050300DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2071944/PubMed CentralArticle
https://doi.org/10.1371/journal.pbio.0060091DOICorrection
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2689690/PubMed CentralCorrection
https://doi.org/10.1371/journal.pbio.0050336DOICorrection
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2222975/PubMed CentralCorrection
ORCID:
AuthorORCID
Kelsch, Wolfgang0000-0002-3470-8125
Lois, Carlos0000-0002-7305-2317
Additional Information:© 2007 Kelsch et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. The project was funded by a grant from the David and Lucille Packard Foundation to CL. Grant support: M.I.T. Newton Fellowship to WK, Singleton M.I.T. Fellowship to CWL, and David and Lucille Packard Foundation to CL. We thank D. Friedmann, E. Hong, and S. Magavi for critically reading the manuscript; S. Song for valuable suggestions regarding statistical analysis; and V. Egger for initial help with the preparation of acute olfactory bulb slices. Author Contributions: WK and CL conceived and designed the experiments and wrote the paper. WK, CPM, and CWL performed the experiments and analyzed the data. WK and CL contributed reagents/materials/analysis tools. The authors have declared that no competing interests exist.
Errata:Kelsch W, Mosley CP, Lin CW, Lois C (2008) Correction: Distinct Mammalian Precursors Are Committed to Generate Neurons with Defined Dendritic Projection Patterns. PLOS Biology 6(4): e91. https://doi.org/10.1371/journal.pbio.0060091 Kelsch W, Mosley CP, Lin CW, Lois C (2007) Correction: Distinct Mammalian Precursors Are Committed to Generate Neurons with Defined Dendritic Projection Patterns. PLOS Biology 5(12): e336. https://doi.org/10.1371/journal.pbio.0050336
Funders:
Funding AgencyGrant Number
David and Lucile Packard FoundationUNSPECIFIED
Massachusetts Institute of Technology (MIT)UNSPECIFIED
Issue or Number:11
PubMed Central ID:PMC2071944
Record Number:CaltechAUTHORS:20181001-113519846
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20181001-113519846
Official Citation:Kelsch W, Mosley CP, Lin C-W, Lois C (2007) Distinct Mammalian Precursors Are Committed to Generate Neurons with Defined Dendritic Projection Patterns. PLoS Biol 5(11): e300. https://doi.org/10.1371/journal.pbio.0050300
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:90057
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:05 Oct 2018 23:02
Last Modified:03 Oct 2019 20:21

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