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Adenovirus-mediated gene delivery into neuronal precursors of the adult mouse brain

Yoon, Sung Ok and Lois, Carlos and Alvirez, Mauricio and Alvarez-Buylla, Arturo and Falck-Pedersen, Erik and Chao, Moses V. (1996) Adenovirus-mediated gene delivery into neuronal precursors of the adult mouse brain. Proceedings of the National Academy of Sciences of the United States of America, 93 (21). pp. 11974-11979. ISSN 0027-8424. PMCID PMC38168. doi:10.1073/pnas.93.21.11974.

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Precursor cells found in the subventricular zone (SVZ) of the adult brain can undergo cell division and migrate long distances before differentiating into mature neurons. We have investigated the possibility of introducing genes stably into this population of cells. Replication-defective adenoviruses were injected into the SVZ of the lateral ventricle of adult mice. The adenoviruses carried a cDNA for the LacZ reporter or the human p75 neurotrophin receptor, for which species-specific antibodies are available. Injection of the viruses into the SVZ led to efficient labeling of neuronal precursors. Two months after viral injection, infected cells were detected in the olfactory bulb, a significant distance from the site of injection. Labeled periglomerular and granular neurons with extensive dendritic arborization were found in the olfactory bulb. These results demonstrate that foreign genes can be efficiently introduced into neuronal precursor cells. Furthermore, adenovirus-directed infection can lead to long-term stable gene expression in progenitor cells found in the adult central nervous system.

Item Type:Article
Related URLs:
URLURL TypeDescription CentralArticle
Lois, Carlos0000-0002-7305-2317
Alvarez-Buylla, Arturo0000-0003-4426-8925
Falck-Pedersen, Erik0000-0002-3327-760X
Chao, Moses V.0000-0002-6969-3744
Additional Information:© 1996 National Academy of Sciences. Communicated by Kenneth I. Berns, Cornell University Medical College, New York, NY, July 31, 1996 (received for review February 7, 1996) We thank B. Bewig and R. Crystal for the LacZ adenovirus. This project was supported by a grant to S.O.Y. by the Multiple Sclerosis Society, grants to M.V.C. from the National Institutes of Health and the Dorothy Rodbell Cohen Foundation, and grants from National Institutes of Health (Program Project HL 51746 to E.F.-P. and HD 32116 to A.A.-B.). The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Funding AgencyGrant Number
Dorothy Rodbell Cohen FoundationUNSPECIFIED
Issue or Number:21
PubMed Central ID:PMC38168
Record Number:CaltechAUTHORS:20181001-113520455
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Official Citation:Adenovirus-mediated gene delivery into neuronal precursors of the adult mouse brain S O Yoon, C Lois, M Alvirez, A Alvarez-Buylla, E Falck-Pedersen, M V Chao Proceedings of the National Academy of Sciences Oct 1996, 93 (21) 11974-11979; DOI: 10.1073/pnas.93.21.11974
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:90062
Deposited By: George Porter
Deposited On:03 Oct 2018 20:44
Last Modified:16 Nov 2021 00:40

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