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Adult Subventricular Zone Neuronal Precursors Continue to Proliferate and Migrate in the Absence of the Olfactory Bulb

Kirschenbaum, Barry and Doetsch, Fiona and Lois, Carlos and Alvarez-Buylla, Arturo (1999) Adult Subventricular Zone Neuronal Precursors Continue to Proliferate and Migrate in the Absence of the Olfactory Bulb. Journal of Neuroscience, 19 (6). pp. 2171-2180. ISSN 0270-6474. PMCID PMC6782582. doi:10.1523/jneurosci.19-06-02171.1999. https://resolver.caltech.edu/CaltechAUTHORS:20181001-113520702

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Abstract

Neurons continue to be born in the subventricular zone (SVZ) of the lateral ventricles of adult mice. These cells migrate as a network of chains through the SVZ and the rostral migratory stream (RMS) into the olfactory bulb (OB), where they differentiate into mature neurons. The OB is the only known target for these neuronal precursors. Here, we show that, after elimination of the OB, the SVZ and RMS persist and become dramatically larger. The proportion of dividing [bromodeoxyuridine (BrdU)-labeled] or dying (pyknotic or terminal deoxynucleotidyl transferase-mediated biotinylated UTP nick end-labeled) cells in the RMS was not significantly affected at 3 d or 3 weeks after bulbectomy (OBX). However, by 3 months after OBX, the percentage of BrdU-labeled cells in the RMS decreased by half and that of dying cells doubled. Surprisingly, the rostral migration of precursors continued along the RMS after OBX. This was demonstrated by focal microinjections of BrdU and grafts of SVZ cells carrying LacZ under the control of a neuron-specific promoter gene. Results indicate that the OB is not essential for proliferation and the directional migration of SVZ precursors.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1523/jneurosci.19-06-02171.1999DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6782582PubMed CentralArticle
ORCID:
AuthorORCID
Doetsch, Fiona0000-0002-0577-1272
Lois, Carlos0000-0002-7305-2317
Alvarez-Buylla, Arturo0000-0003-4426-8925
Additional Information:© 1999 Society for Neuroscience. For the first six months after publication SfN’s license will be exclusive. Beginning six months after publication the Work will be made freely available to the public on SfN’s website to copy, distribute, or display under a Creative Commons Attribution 4.0 International (CC BY 4.0) license (https://creativecommons.org/licenses/by/4.0/). This work was supported by National Institutes of Health Grants HD32116 and NS28478 to A.A.B. and DC03046 to B.K. We thank B. Haripal for help with tissue processing and D. Lim for suggestions on this manuscript.
Funders:
Funding AgencyGrant Number
NIHHD32116
NIHNS28478
NIHDC03046
Subject Keywords:bulbectomy; adult; subventricular zone; neuronal precursors; subependymal layer; cell death
Issue or Number:6
PubMed Central ID:PMC6782582
DOI:10.1523/jneurosci.19-06-02171.1999
Record Number:CaltechAUTHORS:20181001-113520702
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20181001-113520702
Official Citation:Adult Subventricular Zone Neuronal Precursors Continue to Proliferate and Migrate in the Absence of the Olfactory Bulb Barry Kirschenbaum, Fiona Doetsch, Carlos Lois, Arturo Alvarez-Buylla Journal of Neuroscience 15 March 1999, 19 (6) 2171-2180; DOI: 10.1523/JNEUROSCI.19-06-02171.1999
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:90064
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:03 Oct 2018 21:21
Last Modified:16 Nov 2021 00:40

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