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Viable cyanobacteria in the deep continental subsurface

Puente-Sánchez, Fernando and Arce-Rodríguez, Alejandro and Oggerin, Monike and García-Villadangos, Miriam and Moreno-Paz, Mercedes and Blanco Morgado, Yolanda and Rodríguez, Nuria and Bird, Laurence and Lincoln, Sara A. and Tornos, Fernando and Prieto-Ballesteros, Olga and Freeman, Katherine H. and Pieper, Dietmar H. and Timmis, Kenneth N. and Amils, Ricardo and Parro, Víctor (2018) Viable cyanobacteria in the deep continental subsurface. Proceedings of the National Academy of Sciences of the United States of America, 115 (42). pp. 10702-10707. ISSN 0027-8424. https://resolver.caltech.edu/CaltechAUTHORS:20181002-104711126

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Abstract

Cyanobacteria are ecologically versatile microorganisms inhabiting most environments, ranging from marine systems to arid deserts. Although they possess several pathways for light-independent energy generation, until now their ecological range appeared to be restricted to environments with at least occasional exposure to sunlight. Here we present molecular, microscopic, and metagenomic evidence that cyanobacteria predominate in deep subsurface rock samples from the Iberian Pyrite Belt Mars analog (southwestern Spain). Metagenomics showed the potential for a hydrogen-based lithoautotrophic cyanobacterial metabolism. Collectively, our results suggest that they may play an important role as primary producers within the deep-Earth biosphere. Our description of this previously unknown ecological niche for cyanobacteria paves the way for models on their origin and evolution, as well as on their potential presence in current or primitive biospheres in other planetary bodies, and on the extant, primitive, and putative extraterrestrial biospheres.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1073/pnas.1808176115DOIArticle
http://www.pnas.org/lookup/suppl/doi:10.1073/pnas.1808176115/-/DCSupplementalPublisherSupporting Information
ORCID:
AuthorORCID
Puente-Sánchez, Fernando0000-0002-6341-3692
Oggerin, Monike0000-0002-5130-611X
Moreno-Paz, Mercedes0000-0003-1245-3253
Blanco Morgado, Yolanda0000-0002-1834-0498
Freeman, Katherine H.0000-0002-3350-7671
Additional Information:© 2018 the Author(s). Published by PNAS. This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND). Edited by David M. Karl, University of Hawaii, Honolulu, HI, and approved August 29, 2018 (received for review May 11, 2018) PNAS published ahead of print October 1, 2018 https://doi.org/10.1073/pnas.1808176115 We thank Javier Tamames, Carlos Pedrós-Alió, Ramón Roselló-Mora, Barbara Sherwood-Lollar, and Chris McKay for useful feedback and discussions of the draft manuscript and all the members of the Iberian Pyrite Belt Subsurface Life (IPBSL) team and the personnel of the Museo Minero de Riotinto. This work was supported by the European Research Council Advanced Grant ERC250350-IPBSL, and the Spanish Ministerio de Economía y Competitividad/Fondo Europeo de Desarrollo Regional Grants AYA2011-24803 and ESP2015-69540-R. F.P.-S. is currently funded by the Spanish Ministerio de Economía y Competitividad/Fondo Europeo de Desarrollo Regional Grant CTM2016-80095-C2-1-R. Author contributions: F.P.-S., A.A.-R., K.N.T., R.A., and V.P. designed research; F.P.-S., A.A.-R., M.O., M.G.-V., M.M.-P., Y.B., N.R., L.B., S.A.L., F.T., O.P.-B., K.H.F., and D.H.P. performed research; F.P.-S., A.A.-R., M.O., Y.B., F.T., O.P.-B., and V.P. analyzed data; and F.P.-S. wrote the paper. This article is a PNAS Direct Submission. Data deposition: Raw sequence data and assemblies that support the findings of this study have been deposited in NCBI under the BioProject ID PRJNA476489. MG-RAST results are deposited under project ID mgp83581. This article contains supporting information online at www.pnas.org/lookup/suppl/doi:10.1073/pnas.1808176115/-/DCSupplemental. The authors declare no conflict of interest.
Funders:
Funding AgencyGrant Number
European Research Council (ERC)250350
Ministerio de Economía y Competitividad (MINECO)AYA2011-24803
Ministerio de Economía y Competitividad (MINECO)ESP2015-69540-R
Fondo Europeo de Desarrollo Regional (FEDER)UNSPECIFIED
Ministerio de Economía y Competitividad (MINECO)CTM2016-80095-C2-1-R
Subject Keywords:astrobiology | extreme environments | deep/dark biosphere | endolithic cyanobacteria | metagenomics
Issue or Number:42
Record Number:CaltechAUTHORS:20181002-104711126
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20181002-104711126
Official Citation:Viable cyanobacteria in the deep continental subsurface. Fernando Puente-Sánchez, Alejandro Arce-Rodríguez, Monike Oggerin, Miriam García-Villadangos, Mercedes Moreno-Paz, Yolanda Blanco, Nuria Rodríguez, Laurence Bird, Sara A. Lincoln, Fernando Tornos, Olga Prieto-Ballesteros, Katherine H. Freeman, Dietmar H. Pieper, Kenneth N. Timmis, Ricardo Amils, Víctor Parro. Proceedings of the National Academy of Sciences Oct 2018, 115 (42) 10702-10707; DOI: 10.1073/pnas.1808176115
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:90081
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:03 Oct 2018 17:33
Last Modified:09 Mar 2020 13:18

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