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Genetic Labeling of Neuronal Subsets through Enhancer Trapping in Mice

Kelsch, Wolfgang and Stolfi, Alberto and Lois, Carlos (2012) Genetic Labeling of Neuronal Subsets through Enhancer Trapping in Mice. PLoS ONE, 7 (6). Art. No. e38593. ISSN 1932-6203. PMCID PMC3369840.

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The ability to label, visualize, and manipulate subsets of neurons is critical for elucidating the structure and function of individual cell types in the brain. Enhancer trapping has proved extremely useful for the genetic manipulation of selective cell types in Drosophila. We have developed an enhancer trap strategy in mammals by generating transgenic mice with lentiviral vectors carrying single-copy enhancer-detector probes encoding either the marker gene lacZ or Cre recombinase. This transgenic strategy allowed us to genetically identify a wide variety of neuronal subpopulations in distinct brain regions. Enhancer detection by lentiviral transgenesis could thus provide a complementary method for generating transgenic mouse libraries for the genetic labeling and manipulation of neuronal subsets.

Item Type:Article
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URLURL TypeDescription S1 S2 CentralArticle
Kelsch, Wolfgang0000-0002-3470-8125
Lois, Carlos0000-0002-7305-2317
Additional Information:© 2012 Kelsch et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Received: February 23, 2012; Accepted: May 10, 2012; Published: June 7, 2012. This work was funded by an award from the David and Lucille Packard Foundation. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Author Contributions: Conceived and designed the experiments: CL WK AS. Performed the experiments: WK AS. Analyzed the data: CL WK AS. Contributed reagents/materials/analysis tools: CL AS WK. Wrote the paper: WK CL. Ethics Issues: No humans participants were involved in this study. Ethics approval by a committee was not necessary. The experiments involving animals were approved by the IACUC committee of the Massachusetts Institute of Technology. The authors have declared that no competing interests exist.
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David and Lucile Packard FoundationUNSPECIFIED
Issue or Number:6
PubMed Central ID:PMC3369840
Record Number:CaltechAUTHORS:20181002-104711349
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Official Citation:Kelsch W, Stolfi A, Lois C (2012) Genetic Labeling of Neuronal Subsets through Enhancer Trapping in Mice. PLoS ONE 7(6): e38593.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:90083
Deposited By: George Porter
Deposited On:05 Oct 2018 23:02
Last Modified:23 Apr 2020 21:58

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