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Coincident Generation of Pyramidal Neurons and Protoplasmic Astrocytes in Neocortical Columns

Magavi, Sanjay and Friedmann, Drew and Banks, Garrett and Stolfi, Alberto and Lois, Carlos (2012) Coincident Generation of Pyramidal Neurons and Protoplasmic Astrocytes in Neocortical Columns. Journal of Neuroscience, 32 (14). pp. 4762-4772. ISSN 0270-6474. PMCID PMC3643505. https://resolver.caltech.edu/CaltechAUTHORS:20181002-163832137

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Abstract

Astrocytes, one of the most common cell types in the brain, are essential for processes ranging from neural development through potassium homeostasis to synaptic plasticity. Surprisingly, the developmental origins of astrocytes in the neocortex are still controversial. To investigate the patterns of astrocyte development in the neocortex we examined cortical development in a transgenic mouse in which a random, sparse subset of neural progenitors undergoes CRE/lox recombination, permanently labeling their progeny. We demonstrate that neural progenitors in neocortex generate discrete columnar structures that contain both projection neurons and protoplasmic astrocytes. Ninety-five percent of developmental cortical columns labeled in our system contained both astrocytes and neurons. The astrocyte to neuron ratio of labeled cells in a developmental column was 1:7.4, similar to the overall ratio of 1:8.4 across the entire gray matter of the neocortex, indicating that column-associated astrocytes account for the majority of protoplasmic astrocytes in neocortex. Most of the labeled columns contained multiple clusters of several astrocytes. Dividing cells were found at the base of neuronal columns at the beginning of gliogenesis, and later within the cortical layers, suggesting a mechanism by which astrocytes could be distributed within a column. These data indicate that radial glia are the source of both neurons and astrocytes in the neocortex, and that these two cell types are generated in a spatially restricted manner during cortical development.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1523/jneurosci.3560-11.2012DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3643505/PubMed CentralArticle
ORCID:
AuthorORCID
Lois, Carlos0000-0002-7305-2317
Additional Information:© 2012 the authors. For the first six months after publication SfN’s license will be exclusive. Beginning six months after publication the Work will be made freely available to the public on SfN’s website to copy, distribute, or display under a Creative Commons Attribution 4.0 International (CC BY 4.0) license (https://creativecommons.org/licenses/by/4.0/). Received July 11, 2011; revised Jan. 30, 2012; accepted Feb. 9, 2012. This work was supported by the David and Lucile Packard Foundation to C.L.
Funders:
Funding AgencyGrant Number
David and Lucile Packard FoundationUNSPECIFIED
Issue or Number:14
PubMed Central ID:PMC3643505
Record Number:CaltechAUTHORS:20181002-163832137
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20181002-163832137
Official Citation:Coincident Generation of Pyramidal Neurons and Protoplasmic Astrocytes in Neocortical Columns Sanjay Magavi, Drew Friedmann, Garrett Banks, Alberto Stolfi, Carlos Lois Journal of Neuroscience 4 April 2012, 32 (14) 4762-4772; DOI: 10.1523/JNEUROSCI.3560-11.2012
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:90090
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:05 Oct 2018 22:55
Last Modified:03 Oct 2019 20:22

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