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Optical excitation and detection of neuronal activity

Hu, Chenfei and Sam, Richard and Shan, Mingguang and Nastasa, Viorel and Wang, Minqi and Kim, Taewoo and Gillette, Martha and Sengupta, Parijat and Popescu, Gabriel (2019) Optical excitation and detection of neuronal activity. Journal of Biophotonics, 12 (3). Art. No. e201800269. ISSN 1864-063X. http://resolver.caltech.edu/CaltechAUTHORS:20181008-162019678

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Abstract

Optogenetics has emerged as an exciting tool for manipulating neural activity, which in turn, can modulate behavior in live organisms. However, detecting the response to the optical stimulation requires electrophysiology with physical contact or fluorescent imaging at target locations, which is often limited by photobleaching and phototoxicity. In this paper, we show that phase imaging can report the intracellular transport induced by optogenetic stimulation. We developed a multimodal instrument that can both stimulate cells with subcellular spatial resolution and detect optical pathlength changes with nanometer scale sensitivity. We found that optical pathlength fluctuations following stimulation are consistent with active organelle transport. Furthermore, the results indicate a broadening in the transport velocity distribution, which is significantly higher in stimulated cells compared to optogenetically inactive cells. It is likely that this label‐free, contactless measurement of optogenetic response will provide an enabling approach to neuroscience.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1002/jbio.201800269DOIArticle
https://doi.org/10.1101/209759DOIDiscussion Paper
ORCID:
AuthorORCID
Hu, Chenfei0000-0002-9578-966X
Additional Information:© 2018 Wiley. Accepted manuscript online: 12 October 2018; Manuscript accepted: 09 October 2018; Manuscript received: 18 July 2018. We gratefully appreciated the funding support from National Science Foundation (NSF) STC CBET 0939511, NSF BRAIN EAGER DBI 1450962, and IIP-1353368, PS’s start-up funding. Author Contributions: M.G., P.S., and G.P. proposed the project. T.K., M.S., and V.N. built the excitation module. C.H. and M.S built the DPM system. M.W. prepared DNA. R.S. prepared cell samples. C.H. performed quantitative phase imaging and data analysis. G.P. and P.S. supervised the research. C.H, P.S. and G.P. wrote the manuscript with input from all authors. Data availability: The data that support the findings of this study are available from the corresponding author upon reasonable request. Competing financial interest: G.P. has financial interest in Phi Optics, Inc., a company developing quantitative phase imaging technology for materials and life science applications.
Funders:
Funding AgencyGrant Number
NSFCBET-0939511
NSFDBI-1450962
NSFIIP-1353368
University of Illinois Urbana-ChampaignUNSPECIFIED
Subject Keywords:optogenetics; label‐free imaging; cell activation; quantitative phase imaging; intracellular transport
Record Number:CaltechAUTHORS:20181008-162019678
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20181008-162019678
Official Citation:Hu, C, Sam, R, Shan, M, et al. Optical excitation and detection of neuronal activity. J. Biophotonics. 2019; 12:e201800269. https://doi.org/10.1002/jbio.201800269
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:90169
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:09 Oct 2018 14:59
Last Modified:14 Mar 2019 23:27

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