Kaplan, Mohammed and Subramanian, Poorna and Ghosal, Debnath and Oikonomou, Catherine M. and Pirbadian, Sahand and Starwalt-Lee, Ruth and Gralnick, Jeffrey A. and El-Naggar, Mohamed Y. and Jensen, Grant J. (2018) Stable sub-complexes observed in situ suggest a modular assembly pathway of the bacterial flagellar motor. . (Unpublished) https://resolver.caltech.edu/CaltechAUTHORS:20181008-162020064
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Abstract
The self-assembly of cellular macromolecular machines such as the bacterial flagellar motor requires the spatio- temporal synchronization of gene expression, protein localization and association of a dozen or more unique components. In Salmonella and Escherichia coli, a sequential, outward assembly mechanism has been proposed for the flagellar motor starting from the inner membrane, with each subsequent component stabilizing the last. Here, using electron cryo-tomography of intact Legionella pneumophila, Pseudomonas aeruginosa and Shewanella oneidensis cells, we observe stable outer-membrane-embedded sub-complexes of the flagellar motor. These sub- complexes consist of the periplasmic embellished P- and L-rings, in the absence of other flagellar components, and bend the membrane inward dramatically. Additionally, we also observe independent inner-membrane sub- complexes consisting of the C- and MS-rings and export apparatus. These results suggest an alternate model for flagellar motor assembly in which outer- and inner-membrane-associated sub-complexes form independently and subsequently join, enabling later steps of flagellar production to proceed.
Item Type: | Report or Paper (Discussion Paper) | ||||||||||||
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Additional Information: | The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license. This work was supported by the National Institute of Health (NIH, grant R01 AI127401 to G.J.J.). M.K. is supported by a postdoctoral Rubicon fellowship from De Nederlandse Organisatie voor Wetenschappelijk Onderzoek (NWO). S.P. and M.Y.E.-N. are supported by the Air Force Office of Scientific Research Presidential Early Career Award for Scientists and Engineers (FA955014-1-0294, to M.Y.E.-N.). | ||||||||||||
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Record Number: | CaltechAUTHORS:20181008-162020064 | ||||||||||||
Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20181008-162020064 | ||||||||||||
Official Citation: | Stable sub-complexes observed in situ suggest a modular assembly pathway of the bacterial flagellar motor Mohammed Kaplan, Poorna Subramanian, Debnath Ghosal, Catherine M Oikonomou, Sahand Pirbadian, Ruth Starwalt-Lee, Jeffrey A Gralnick, Mohamed Y El-Naggar, Grant J Jensen bioRxiv 369405; doi: https://doi.org/10.1101/369405 | ||||||||||||
Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||||||||
ID Code: | 90172 | ||||||||||||
Collection: | CaltechAUTHORS | ||||||||||||
Deposited By: | George Porter | ||||||||||||
Deposited On: | 09 Oct 2018 14:56 | ||||||||||||
Last Modified: | 03 Oct 2019 20:22 |
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