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Stable sub-complexes observed in situ suggest a modular assembly pathway of the bacterial flagellar motor

Kaplan, Mohammed and Subramanian, Poorna and Ghosal, Debnath and Oikonomou, Catherine M. and Pirbadian, Sahand and Starwalt-Lee, Ruth and Gralnick, Jeffrey A. and El-Naggar, Mohamed Y. and Jensen, Grant J. (2018) Stable sub-complexes observed in situ suggest a modular assembly pathway of the bacterial flagellar motor. . (Unpublished) https://resolver.caltech.edu/CaltechAUTHORS:20181008-162020064

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Abstract

The self-assembly of cellular macromolecular machines such as the bacterial flagellar motor requires the spatio- temporal synchronization of gene expression, protein localization and association of a dozen or more unique components. In Salmonella and Escherichia coli, a sequential, outward assembly mechanism has been proposed for the flagellar motor starting from the inner membrane, with each subsequent component stabilizing the last. Here, using electron cryo-tomography of intact Legionella pneumophila, Pseudomonas aeruginosa and Shewanella oneidensis cells, we observe stable outer-membrane-embedded sub-complexes of the flagellar motor. These sub- complexes consist of the periplasmic embellished P- and L-rings, in the absence of other flagellar components, and bend the membrane inward dramatically. Additionally, we also observe independent inner-membrane sub- complexes consisting of the C- and MS-rings and export apparatus. These results suggest an alternate model for flagellar motor assembly in which outer- and inner-membrane-associated sub-complexes form independently and subsequently join, enabling later steps of flagellar production to proceed.


Item Type:Report or Paper (Discussion Paper)
Related URLs:
URLURL TypeDescription
https://doi.org/10.1101/369405DOIDiscussion Paper
ORCID:
AuthorORCID
Kaplan, Mohammed0000-0002-0759-0459
Ghosal, Debnath0000-0002-2227-0330
Oikonomou, Catherine M.0000-0003-2312-4746
Gralnick, Jeffrey A.0000-0001-9250-7770
Jensen, Grant J.0000-0003-1556-4864
Additional Information:The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license. This work was supported by the National Institute of Health (NIH, grant R01 AI127401 to G.J.J.). M.K. is supported by a postdoctoral Rubicon fellowship from De Nederlandse Organisatie voor Wetenschappelijk Onderzoek (NWO). S.P. and M.Y.E.-N. are supported by the Air Force Office of Scientific Research Presidential Early Career Award for Scientists and Engineers (FA955014-1-0294, to M.Y.E.-N.).
Funders:
Funding AgencyGrant Number
NIHR01 AI127401
Nederlandse Organisatie voor Wetenschappelijk Onderzoek (NWO)UNSPECIFIED
Air Force Office of Scientific Research (AFOSR)FA955014-1-0294
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Record Number:CaltechAUTHORS:20181008-162020064
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20181008-162020064
Official Citation:Stable sub-complexes observed in situ suggest a modular assembly pathway of the bacterial flagellar motor Mohammed Kaplan, Poorna Subramanian, Debnath Ghosal, Catherine M Oikonomou, Sahand Pirbadian, Ruth Starwalt-Lee, Jeffrey A Gralnick, Mohamed Y El-Naggar, Grant J Jensen bioRxiv 369405; doi: https://doi.org/10.1101/369405
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:90172
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:09 Oct 2018 14:56
Last Modified:03 Oct 2019 20:22

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