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The expanded universe of prokaryotic Argonaute proteins

Ryazansky, Sergei and Kulbachinskiy, Andrey and Aravin, Alexei A. (2018) The expanded universe of prokaryotic Argonaute proteins. mBio, 9 (6). Art. No. e01935-18. ISSN 2150-7511. PMCID PMC6299218. https://resolver.caltech.edu/CaltechAUTHORS:20181008-162020161

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Abstract

Members of the ancient family of Argonaute (Ago) proteins are present in all domains of life. The common feature of Ago proteins is the ability to bind small nucleic acid guides and use them for sequence-specific recognition—and sometimes cleavage—of complementary targets. While eukaryotic Ago (eAgo) proteins are key players in RNA interference and related pathways, the properties and functions of these proteins in archaeal and bacterial species have just started to emerge. We undertook comprehensive exploration of prokaryotic Ago (pAgo) proteins in sequenced genomes and revealed their striking diversity in comparison with eAgos. Many pAgos contain divergent variants of the conserved domains involved in interactions with nucleic acids, while having extra domains that are absent in eAgos, suggesting that they might have unusual specificities in the nucleic acid recognition and cleavage. Many pAgos are associated with putative nucleases, helicases, and DNA binding proteins in the same gene or operon, suggesting that they are involved in target processing. The great variability of pAgos revealed by our analysis opens new ways for exploration of their functions in host cells and for their use as potential tools in genome editing.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1128/mBio.01935-18UNSPECIFIEDArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6299218/PubMed CentralArticle
https://doi.org/10.1101/366930DOIDiscussion Paper
ORCID:
AuthorORCID
Ryazansky, Sergei0000-0002-3171-2997
Kulbachinskiy, Andrey0000-0002-2292-6424
Additional Information:© 2018 Ryazansky et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license. Received 3 September 2018; Accepted 20 November 2018; Published 18 December 2018. We thank Mindaugas Margelevičius for the help with the prediction of PD-(D/E)XK motifs in proteins. This work was supported by the grant of the Ministry of Education and Science of the Russian Federation 14.W03.31.0007.
Funders:
Funding AgencyGrant Number
Ministry of Education and Science of Russian Federation14.W03.31.0007
Subject Keywords:Ago, RNA interference, genome editing, horizontal gene transfer, prokaryotic Argonaute proteins
Issue or Number:6
PubMed Central ID:PMC6299218
Record Number:CaltechAUTHORS:20181008-162020161
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20181008-162020161
Official Citation:The Expanded Universe of Prokaryotic Argonaute Proteins. Sergei Ryazansky, Andrey Kulbachinskiy, Alexei A. Aravin mBio Dec 2018, 9 (6) e01935-18; DOI: 10.1128/mBio.01935-18
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:90173
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:09 Oct 2018 14:49
Last Modified:03 Oct 2019 20:22

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