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In situ gene expression analysis of mucoid P. aeruginosa biofilms suggests that alginate expression is restricted to microaerobic regions of the biofilm

Jorth, P. and Spero, M. A. and Newman, D. K. (2018) In situ gene expression analysis of mucoid P. aeruginosa biofilms suggests that alginate expression is restricted to microaerobic regions of the biofilm. Pediatric Pulmonology, 53 (S2). Art. No. 462. ISSN 8755-6863. https://resolver.caltech.edu/CaltechAUTHORS:20181009-123252928

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Abstract

Mucoid Pseudomonas aeruginosa evolve during chronic cystic fibrosis (CF) infections and make robust biofilms that protect against antibiotics and the immune response. Previous measurements suggested that alginate gene expression is induced in response to low oxygen, however, these measurements were made in bulk liquid cultures. Because P. aeruginosa is thought to grow as biofilms in vivo and biofilms are known to exhibit large oxygen gradients, we wondered whether alginate production within biofilms might be localized to particular regions. We used confocal microscopy to determine where alginate transcripts are expressed within biofilm populations grown using the agar block biofilm assay (ABBA), which permits rapid study of biofilms; the ABBA assay establishes oxygen gradients over the entire aggregate population and within individual aggregates.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1002/ppul.24152DOIAbstracts
ORCID:
AuthorORCID
Jorth, P.0000-0002-0981-740X
Spero, M. A.0000-0003-3291-2138
Newman, D. K.0000-0003-1647-1918
Additional Information:© 2018 Wiley Periodicals, Inc. First published: 07 September 2018.
Issue or Number:S2
Record Number:CaltechAUTHORS:20181009-123252928
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20181009-123252928
Official Citation:(2018), Poster Session Abstracts. Pediatr Pulmonol, 53: S148-S456. doi:10.1002/ppul.24152
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:90193
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:10 Oct 2018 17:57
Last Modified:20 Dec 2019 17:20

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