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FRET-Based Real-Time DNA Microarrays

Hassibi, Arjang and Vikalo, Haris and Riechmann, José Luis and Hassibi, Babak (2011) FRET-Based Real-Time DNA Microarrays. In: Functional Genomics: Methods and Protocols. Methods in Molecular Biology. No.815. Springer , New York, NY, pp. 147-159. ISBN 978-1-61779-423-0.

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We present a quantification method for affinity-based DNA microarrays which is based on the real-time measurements of hybridization kinetics. This method, i.e., real-time DNA microarrays, enhances the detection dynamic range of conventional systems by being impervious to probe saturation, washing artifacts, microarray spot-to-spot variations, and other intensity-affecting impediments. We demonstrate in both theory and practice that the time-constant of target capturing is inversely proportional to the concentration of the target analyte, which we take advantage of as the fundamental parameter to estimate the concentration of the analytes. Furthermore, to experimentally validate the capabilities of this method in practical applications, we present a FRET-based assay which enables the real-time detection in gene expression DNA microarrays.

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Additional Information:© 2012 Springer Science+Business Media, LLC. First Online: 04 November 2011. We are grateful to Vijaya Kumar for experimental assistance with microarray manufacture and target labeling. We also want to thank Professor Scott Fraser at Caltech for technical feedback in the imaging aspects of this project.
Subject Keywords:Microarray; Real-time; Gene expression; Time-constant; FRET
Series Name:Methods in Molecular Biology
Issue or Number:815
Record Number:CaltechAUTHORS:20181010-162103240
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:90241
Deposited By: Tony Diaz
Deposited On:11 Oct 2018 06:05
Last Modified:03 Oct 2019 20:23

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