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Genetic Labeling of Synapses

Lois, Carlos and Kelsch, Wolfgang (2014) Genetic Labeling of Synapses. In: Neural Tracing Methods: Tracing Neurons and Their Connections. Neuromethods. No.92. Humana Press , New York, NY, pp. 231-248. ISBN 978-1-4939-1962-8.

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A major challenge in neuroscience is to unravel how the synaptic contacts between neurons give rise to brain circuits. A number of techniques have been developed to visualize the synaptic organization of neurons. In this chapter, we focus on genetic methods to mark specific types of synapses so that synaptic sites can be visualized throughout the entire dendritic or axonal arbor of single neurons. Genetic synaptic labeling can be achieved by cell-type-specific viral or transgenic delivery of synaptic proteins tagged by fluorescent proteins. Sparse genetic labeling of neurons permits semiautomated quantification of the distribution and densities of selected types of synapses in segregated domains of the axonal and dendritic trees. These approaches can reduce the complexity and ambiguity of attributing synaptic sites to unravel principles of the synaptic organization of identified neuronal types in the circuit.

Item Type:Book Section
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URLURL TypeDescription
Lois, Carlos0000-0002-7305-2317
Kelsch, Wolfgang0000-0002-3470-8125
Additional Information:© 2015 Springer Science+Business Media New York. First Online: 21 October 2014.
Subject Keywords:Synaptic organization; Synaptic markers; Neurons; Genetic synaptic labeling; Synaptophysin; PSD-95; Retroviruses
Series Name:Neuromethods
Issue or Number:92
Record Number:CaltechAUTHORS:20181012-091948781
Persistent URL:
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:90261
Deposited By: Tony Diaz
Deposited On:12 Oct 2018 20:23
Last Modified:16 Nov 2021 03:31

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