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High-speed widefield photoacoustic microscopy of small-animal hemodynamics

Lan, Bangxin and Liu, Wei and Wang, Ya-chao and Shi, Junhui and Li, Yang and Xu, Song and Sheng, Huaxin and Zhou, Qifa and Zou, Jun and Hoffmann, Ulrike and Yang, Wei and Yao, Junjie (2018) High-speed widefield photoacoustic microscopy of small-animal hemodynamics. Biomedical Optics Express, 9 (10). pp. 4689-4701. ISSN 2156-7085. PMCID PMC6179413. https://resolver.caltech.edu/CaltechAUTHORS:20181016-091038256

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Abstract

Optical-resolution photoacoustic microscopy (OR-PAM) has become a popular tool in small-animal hemodynamic studies. However, previous OR-PAM techniques variously lacked a high imaging speed and/or a large field of view, impeding the study of highly dynamic physiologic and pathophysiologic processes over a large region of interest. Here we report a high-speed OR-PAM system with an ultra-wide field of view, enabled by an innovative water-immersible hexagon-mirror scanner. By driving the hexagon-mirror scanner with a high-precision DC motor, the new OR-PAM has achieved a cross-sectional frame rate of 900 Hz over a 12-mm scanning range, which is 3900 times faster than our previous motor-scanner-based system and 10 times faster than the MEMS-scanner-based system. Using this hexagon-scanner-based OR-PAM system, we have imaged epinephrine-induced vasoconstriction in the whole mouse ear and vascular reperfusion after ischemic stroke in the mouse cortex in vivo, with a high spatial resolution and high volumetric imaging speed. We expect that the hexagon-scanner-based OR-PAM system will become a powerful tool for small animal imaging where the hemodynamic responses over a large field of view are of interest.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1364/boe.9.004689DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6179413PubMed CentralArticle
ORCID:
AuthorORCID
Liu, Wei0000-0002-1727-6738
Shi, Junhui0000-0002-5741-2781
Li, Yang0000-0002-4939-8174
Zou, Jun0000-0002-9543-6135
Additional Information:© 2018 Optical Society of America under the terms of the OSA Open Access Publishing Agreement. Received 25 Jul 2018; revised 3 Sep 2018; accepted 4 Sep 2018; published 7 Sep 2018. Funding: NIH (NS099590 and NS097554); AHA (18CSA34080277); Duke University MEDx fund. We would like to acknowledge the support of NIH grants NS099590 and NS097554 (to W.Y.), AHA collaborative sciences grant 18CSA34080277 (to W.Y., J.Y., and U.H.), and Duke University MEDx fund (to J.Y.). The authors declare that there are no conflicts of interest related to this article.
Funders:
Funding AgencyGrant Number
NIHNS099590
NIHNS097554
American Heart Association18CSA34080277
Duke UniversityUNSPECIFIED
Issue or Number:10
PubMed Central ID:PMC6179413
Record Number:CaltechAUTHORS:20181016-091038256
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20181016-091038256
Official Citation:Bangxin Lan, Wei Liu, Ya-chao Wang, Junhui Shi, Yang Li, Song Xu, Huaxin Sheng, Qifa Zhou, Jun Zou, Ulrike Hoffmann, Wei Yang, and Junjie Yao, "High-speed widefield photoacoustic microscopy of small-animal hemodynamics," Biomed. Opt. Express 9, 4689-4701 (2018)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:90275
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:16 Oct 2018 17:18
Last Modified:09 Mar 2020 13:19

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