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Engineered resistance to Zika virus in transgenic Aedes aegypti expressing a polycistronic cluster of synthetic small RNAs

Buchman, Anna and Gamez, Stephanie and Li, Ming and Antoshechkin, Igor and Li, Hsing-Han and Wang, Hsin-Wei and Chen, Chun-Hong and Klein, Melissa J. and Duchmemin, Jean-Bernard and Paradkar, Prasad N. and Akbari, Omar S. (2019) Engineered resistance to Zika virus in transgenic Aedes aegypti expressing a polycistronic cluster of synthetic small RNAs. Proceedings of the National Academy of Sciences of the United States of America, 116 (9). pp. 3656-3661. ISSN 0027-8424. PMCID PMC6397566. https://resolver.caltech.edu/CaltechAUTHORS:20181030-080547581

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Abstract

Recent Zika virus (ZIKV) outbreaks have highlighted the necessity for development of novel vector control strategies to combat arboviral transmission, including genetic versions of the sterile insect technique, artificial infection with Wolbachia to reduce population size and/or vectoring competency, and gene drive-based methods. Here, we describe the development of mosquitoes synthetically engineered to impede vector competence to ZIKV. We demonstrate that a polycistronic cluster of engineered synthetic small RNAs targeting ZIKV is expressed and fully processed in Aedes aegypti, ensuring the formation of mature synthetic small RNAs in the midgut where ZIKV resides in the early stages of infection. Critically, we demonstrate that engineered Ae. aegypti mosquitoes harboring the anti-ZIKV transgene have significantly reduced viral infection, dissemination, and transmission rates of ZIKV. Taken together, these compelling results provide a promising path forward for development of effective genetic-based ZIKV control strategies, which could potentially be extended to curtail other arboviruses.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1073/pnas.1810771116DOIArticle
https://www.pnas.org/content/suppl/2019/02/04/1810771116.DCSupplementalPublisherSupporting Information
https://doi.org/10.1101/344697DOIDiscussion Paper
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6397566PubMed CentralArticle
ORCID:
AuthorORCID
Buchman, Anna0000-0002-8775-6147
Gamez, Stephanie0000-0003-0665-0066
Antoshechkin, Igor0000-0002-9934-3040
Akbari, Omar S.0000-0002-6853-9884
Alternate Title:Engineered resistance to Zika virus in transgenic Ae. aegypti expressing a polycistronic cluster of synthetic miRNAs
Additional Information:© 2019 the Author(s). Published by PNAS. This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND). Edited by Alexander S. Raikhel, University of California, Riverside, CA, and approved December 31, 2018 (received for review June 22, 2018) We thank Prof. Robert Tesh and Dr. Nikos Vasilakis (University of Texas Medical Branch) for providing the ZIKV FSS13025 and PRVABC59 strains and Lee Trinidad for preparing viral stocks. We thank Christian Bowman for helping with the inverse PCR assay. We also thank Prof. Scott O’Neill (Institute of Vector-Borne Diseases, Monash University) and the World Mosquito Program for providing Wolbachia-infected mosquito eggs. Finally, we thank Dr. Guann-Yi Yu (National Tsing Hua University) for providing the Stat^(−/−) mice used in this study. This work was supported, in part, by an NIH-K22 Career Transition Award (5K22AI113060), an NIH Exploratory/Developmental Research Grant Award (1R21AI123937 to O.S.A.), and CSIRO internal funding (to P.N.P.). A.B. and S.G. contributed equally to this work. Author contributions: A.B., P.N.P., and O.S.A. conceived and designed experiments; A.B., S.G., and M.L. performed all molecular and genetic experiments; I.A. performed sequencing and bioinformatic analysis; M.J.K., J.-B.D., and P.N.P. performed and analyzed data for ZIKV mosquito challenge assays; H.-H.L., H.-W.W., and C.-H.C. performed and analyzed data for in vivo mouse model ZIKV mosquito challenge assays; and A.B., S.G., M.L., I.A., H.-H.L., H.-W.W., C.-H.C., M.J.K., J.-B.D., P.N.P., and O.S.A. wrote the paper. Conflict of interest statement: A.B. and O.S.A have submitted a provisional patent application on this technology. All other authors declare no competing financial interests. This article is a PNAS Direct Submission. Data deposition: All sequencing data associated with this study are available from the National Center for Biotechnology Information Sequence Read Archive (accession no. SRP150144, BioProject accession no. PRJNA475410). The complete annotated plasmid sequence and DNA are publicly available at Addgene (plasmid no. 104968). This article contains supporting information online at www.pnas.org/lookup/suppl/doi:10.1073/pnas.1810771116/-/DCSupplemental.
Funders:
Funding AgencyGrant Number
NIH5K22AI113060
NIH1R21AI123937
Commonwealth Scientific and Industrial Research Organisation (CSIRO)UNSPECIFIED
Subject Keywords:ZIKA; resistance; Aedes; aegypti; mosquito
Issue or Number:9
PubMed Central ID:PMC6397566
Record Number:CaltechAUTHORS:20181030-080547581
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20181030-080547581
Official Citation:Engineered resistance to Zika virus in transgenic Aedes aegypti expressing a polycistronic cluster of synthetic small RNAs. Anna Buchman, Stephanie Gamez, Ming Li, Igor Antoshechkin, Hsing-Han Li, Hsin-Wei Wang, Chun-Hong Chen, Melissa J. Klein, Jean-Bernard Duchemin, Prasad N. Paradkar, Omar S. Akbari. Proceedings of the National Academy of Sciences Feb 2019, 116 (9) 3656-3661; DOI: 10.1073/pnas.1810771116
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:90488
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:30 Oct 2018 17:07
Last Modified:09 Mar 2020 13:18

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