CaltechAUTHORS
  A Caltech Library Service

Optogenetic manipulation of medullary neurons in the locust optic lobe

Wang, Hongxia and Dewell, Richard B. and Ehrengruber, Markus U. and Segev, Eran and Reimer, Jacob and Roukes, Michael L. and Gabbiani, Fabrizio (2018) Optogenetic manipulation of medullary neurons in the locust optic lobe. Journal of Neurophysiology, 120 (4). pp. 2049-2058. ISSN 0022-3077. PMCID PMC6230808. https://resolver.caltech.edu/CaltechAUTHORS:20181030-131638501

[img] PDF - Submitted Version
See Usage Policy.

17Mb

Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20181030-131638501

Abstract

The locust is a widely used animal model for studying sensory processing and its relation to behavior. Due to the lack of genomic information, genetic tools to manipulate neural circuits in locusts are not yet available. We examined whether Semliki Forest virus is suitable to mediate exogenous gene expression in neurons of the locust optic lobe. We subcloned a channelrhodopsin variant and the yellow fluorescent protein Venus into a Semliki Forest virus vector and injected the virus into the optic lobe of locusts (Schistocerca americana). Fluorescence was observed in all injected optic lobes. Most neurons that expressed the recombinant proteins were located in the first two neuropils of the optic lobe, the lamina and medulla. Extracellular recordings demonstrated that laser illumination increased the firing rate of medullary neurons expressing channelrhodopsin. The optogenetic activation of the medullary neurons also triggered excitatory postsynaptic potentials and firing of a postsynaptic, looming-sensitive neuron, the lobula giant movement detector. These results indicate that Semliki Forest virus is efficient at mediating transient exogenous gene expression and provides a tool to manipulate neural circuits in the locust nervous system and likely other insects.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1152/jn.00356.2018DOIArticle
https://doi.org/10.1101/334003DOIDiscussion Paper
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6230808/PubMed CentralArticle
ORCID:
AuthorORCID
Dewell, Richard B.0000-0003-2430-8184
Roukes, Michael L.0000-0002-2916-6026
Gabbiani, Fabrizio0000-0003-4966-3027
Additional Information:© 2018 the American Physiological Society. Received 31 May 2018; Accepted 9 August 2018; Published online 9 October 2018; Published in print 1 October 2018. We thank Drs. Hiromu Yawo and Toru Ishizuka for providing pChopWR-Venus. We also thank Drs. Emma Victoria Jones and Keith Murai for providing the plasmids pENTR2B and pScaA7-RFA, and Dr. Alan L. Goldin for the pSFV-Helper2. This work was supported by a grant from the National Institute of Mental Health (MH-065339) and the National Science Foundation (IIS-1607518). No conflicts of interest, financial or otherwise, are declared by the authors. Author Contributions: H.W., M.U.E., J.R., M.L.R., and F.G. conceived and designed research; H.W., R.B.D., E.S., and J.R. performed experiments; H.W. and R.B.D. analyzed data; H.W., R.B.D., and F.G. interpreted results of experiments; H.W. and F.G. prepared figures; H.W. and F.G. drafted manuscript; H.W., R.B.D., M.U.E., and F.G. edited and revised manuscript; H.W., R.B.D., M.U.E., E.S., J.R., M.L.R., and F.G. approved final version of manuscript.
Funders:
Funding AgencyGrant Number
NIHMH-065339
NSFIIS-1607518
Subject Keywords:LGMD; locust; medulla; optogenetics; Semliki Forest virus
Issue or Number:4
PubMed Central ID:PMC6230808
Record Number:CaltechAUTHORS:20181030-131638501
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20181030-131638501
Official Citation:Optogenetic manipulation of medullary neurons in the locust optic lobe. Hongxia Wang, Richard B. Dewell, Markus U. Ehrengruber, Eran Segev, Jacob Reimer, Michael L. Roukes, and Fabrizio Gabbiani. Journal of Neurophysiology 2018 120:4, 2049-2058
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:90505
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:30 Oct 2018 20:31
Last Modified:17 Jan 2020 22:15

Repository Staff Only: item control page