Wimberly, Brian T. and Brodersen, Ditlev E. and Clemons, William M., Jr. and Morgan-Warren, Robert J. and Carter, Andrew P. and Vonrhein, Clemens and Hartsch, Thomas and Ramakrishnan, V. (2000) Structure of the 30S ribosomal subunit. Nature, 407 (6802). pp. 327-339. ISSN 0028-0836. doi:10.1038/35030006. https://resolver.caltech.edu/CaltechAUTHORS:20181030-134119080
Full text is not posted in this repository. Consult Related URLs below.
Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20181030-134119080
Abstract
Genetic information encoded in messenger RNA is translated into protein by the ribosome, which is a large nucleoprotein complex comprising two subunits, denoted 30S and 50S in bacteria. Here we report the crystal structure of the 30S subunit from Thermus thermophilus, refined to 3 Å resolution. The final atomic model rationalizes over four decades of biochemical data on the ribosome, and provides a wealth of information about RNA and protein structure, protein–RNA interactions and ribosome assembly. It is also a structural basis for analysis of the functions of the 30S subunit, such as decoding, and for understanding the action of antibiotics. The structure will facilitate the interpretation in molecular terms of lower resolution structural data on several functional states of the ribosome from electron microscopy and crystallography.
Item Type: | Article | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
Related URLs: |
| ||||||||||
ORCID: |
| ||||||||||
Additional Information: | © 2000 Nature Publishing Group. Received 14 July; accepted 10 August 2000. This work was supported by the Medical Research Council (UK) and a US National Institutes of Health grant to V.R. and S. W. White. Beamlines at Argonne and Brookhaven were supported by the US Department of Energy. D.E.B. was supported by an EMBO long-term postdoctoral fellowship,and W.M.C. by an NIH predoctoral fellowship. We thank B. S. Brunschwig and M. H. Chou for gifts of osmium hexammine and osmium bipyridine; T. Terwilliger for help with phasing using SOLVE; T. A. Leaf-Jones for providing us a version of O with RNA tools; and our colleagues at the LMB for their advice and encouragement. We are indebted to A. Joachimiak, S. L. Ginell, R. Ravelli, S. McSweeney, G. Leonard, A. Thompson, H. Lewis, L. Berman, M. Papiz, S. Girdwood and M. MacDonald for help and advice on synchrotron beamlines. | ||||||||||
Funders: |
| ||||||||||
Issue or Number: | 6802 | ||||||||||
DOI: | 10.1038/35030006 | ||||||||||
Record Number: | CaltechAUTHORS:20181030-134119080 | ||||||||||
Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20181030-134119080 | ||||||||||
Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||||||
ID Code: | 90508 | ||||||||||
Collection: | CaltechAUTHORS | ||||||||||
Deposited By: | George Porter | ||||||||||
Deposited On: | 31 Oct 2018 14:10 | ||||||||||
Last Modified: | 16 Nov 2021 03:33 |
Repository Staff Only: item control page