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Structural Basis for the Action of the Antibiotics Tetracycline, Pactamycin, and Hygromycin B on the 30S Ribosomal Subunit

Brodersen, Ditlev E. and Clemons, William M., Jr. and Carter, Andrew P. and Morgan-Warren, Robert J. and Wimberly, Brian T. and Ramakrishnan, V. (2000) Structural Basis for the Action of the Antibiotics Tetracycline, Pactamycin, and Hygromycin B on the 30S Ribosomal Subunit. Cell, 103 (7). pp. 1143-1154. ISSN 0092-8674. http://resolver.caltech.edu/CaltechAUTHORS:20181030-134119286

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Abstract

We have used the recently determined atomic structure of the 30S ribosomal subunit to determine the structures of its complexes with the antibiotics tetracycline, pactamycin, and hygromycin B. The antibiotics bind to discrete sites on the 30S subunit in a manner consistent with much but not all biochemical data. For each of these antibiotics, interactions with the 30S subunit suggest a mechanism for its effects on ribosome function.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1016/s0092-8674(00)00216-6DOIArticle
ORCID:
AuthorORCID
Clemons, William M., Jr.0000-0002-0021-889X
Additional Information:© 2000 Cell Press. Under an Elsevier user license. Received 14 November 2000, Revised 4 December 2000. This work was supported by the Medical Research Council (UK). D. E. B. was supported by a Human Frontiers (HFSP) long-term postdoctoral fellowship and W. M. C. by an NIH predoctoral fellowship. We thank Professor A. S. Mankin for help in obtaining pactamycin, and the Drug Synthesis & Chemistry Branch, Developmental Therapeutics Program, Division of Cancer Treatment and Diagnosis, National Cancer Institute, US National Institutes of Health for supplying the compound. We also thank R. Ravelli, S. McSweeney, and G. Leonard for help and advice with synchrotron data collection at the European Synchrotron Radiation Facility (ESRF), Grenoble, France. Protein Data Bank ID Codes: Coordinates have been deposited in the RCSB protein data bank with accession codes 1HNW (Tc), 1HNX (Pct), and 1HNZ (HygB).
Funders:
Funding AgencyGrant Number
Medical Research Council (UK)UNSPECIFIED
Human Frontier Science ProgramUNSPECIFIED
NIH Predoctoral FellowshipUNSPECIFIED
Record Number:CaltechAUTHORS:20181030-134119286
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20181030-134119286
Official Citation:Ditlev E. Brodersen, William M. Clemons, Andrew P. Carter, Robert J. Morgan-Warren, Brian T. Wimberly, V. Ramakrishnan, The Structural Basis for the Action of the Antibiotics Tetracycline, Pactamycin, and Hygromycin B on the 30S Ribosomal Subunit, Cell, Volume 103, Issue 7, 2000, Pages 1143-1154, ISSN 0092-8674, https://doi.org/10.1016/S0092-8674(00)00216-6. (http://www.sciencedirect.com/science/article/pii/S0092867400002166)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:90510
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:30 Oct 2018 23:15
Last Modified:30 Oct 2018 23:15

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