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Crystal Structure of an Initiation Factor Bound to the 30S Ribosomal Subunit

Carter, Andrew P. and Clemons, William M., Jr. and Brodersen, Detlev E. and Morgan-Warren, Robert J. and Hartsch, Thomas and Wimberly, Brian T. and Ramakrishnan, V. (2001) Crystal Structure of an Initiation Factor Bound to the 30S Ribosomal Subunit. Science, 291 (5503). pp. 498-501. ISSN 0036-8075. https://resolver.caltech.edu/CaltechAUTHORS:20181030-134119382

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Abstract

Initiation of translation at the correct position on messenger RNA is essential for accurate protein synthesis. In prokaryotes, this process requires three initiation factors: IF1, IF2, and IF3. Here we report the crystal structure of a complex of IF1 and the 30S ribosomal subunit. Binding of IF1 occludes the ribosomal A site and flips out the functionally important bases A1492 and A1493 from helix 44 of 16S RNA, burying them in pockets in IF1. The binding of IF1 causes long-range changes in the conformation of H44 and leads to movement of the domains of 30S with respect to each other. The structure explains how localized changes at the ribosomal A site lead to global alterations in the conformation of the 30S subunit.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1126/science.1057766DOIArticle
ORCID:
AuthorORCID
Clemons, William M., Jr.0000-0002-0021-889X
Additional Information:© 2001 American Association for the Advancement of Science. 27 November 2000; accepted 20 December 2000. We thank M. Pacold and J. Ogle for critical comments on the manuscript and R. Ravelli, S. McSweeney, and G. Leonard for help and advice during synchrotron data collection at ESRF, Grenoble, France. Supported by the Medical Research Council (U.K.) and grant GM 44973 from the NIH (S. W. White and V.R.) and by a Human Frontier Science Program long-term postdoctoral fellowship (D.E.B.) and an NIH predoctoral fellowship (W.M.C.). Coordinates have been deposited in the Protein Data Bank with the accession number 1HR0.
Funders:
Funding AgencyGrant Number
Medical Research Council (UK)UNSPECIFIED
NIHGM44973
Human Frontier Science ProgramUNSPECIFIED
NIH Predoctoral FellowshipUNSPECIFIED
Issue or Number:5503
Record Number:CaltechAUTHORS:20181030-134119382
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20181030-134119382
Official Citation:Crystal Structure of an Initiation Factor Bound to the 30S Ribosomal Subunit BY ANDREW P. CARTER, WILLIAM M. CLEMONS JR., DITLEV E. BRODERSEN, ROBERT J. MORGAN-WARREN, THOMAS HARTSCH, BRIAN T. WIMBERLY, V. RAMAKRISHNAN Science 19 Jan 2001: Vol. 291, Issue 5503, pp. 498-501 DOI: 10.1126/science.1057766
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:90511
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:30 Oct 2018 23:10
Last Modified:03 Oct 2019 20:26

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