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Recognition of Cognate Transfer RNA by the 30S Ribosomal Subunit

Ogle, James M. and Brodersen, Ditlev E. and Clemons, William M., Jr. and Tarry, Michael J. and Carter, Andrew P. and Ramakrishnan, V. (2001) Recognition of Cognate Transfer RNA by the 30S Ribosomal Subunit. Science, 292 (5518). pp. 897-902. ISSN 0036-8075. https://resolver.caltech.edu/CaltechAUTHORS:20181030-134119461

[img] Image (GIF) (Supplemental Animation 1. Animated GIF file showing relative domain movements in the 16S rRNA of the 30S subunit) - Supplemental Material
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Abstract

Crystal structures of the 30S ribosomal subunit in complex with messenger RNA and cognate transfer RNA in the A site, both in the presence and absence of the antibiotic paromomycin, have been solved at between 3.1 and 3.3 angstroms resolution. Cognate transfer RNA (tRNA) binding induces global domain movements of the 30S subunit and changes in the conformation of the universally conserved and essential bases A1492, A1493, and G530 of 16S RNA. These bases interact intimately with the minor groove of the first two base pairs between the codon and anticodon, thus sensing Watson-Crick base-pairing geometry and discriminating against near-cognate tRNA. The third, or “wobble,” position of the codon is free to accommodate certain noncanonical base pairs. By partially inducing these structural changes, paromomycin facilitates binding of near-cognate tRNAs.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1126/science.1060612DOIArticle
http://science.sciencemag.org/content/suppl/2001/05/02/292.5518.897.DC1PublisherSupporting Information
ORCID:
AuthorORCID
Clemons, William M., Jr.0000-0002-0021-889X
Additional Information:© 2001 American Association for the Advancement of Science. 12 March 2001; accepted 3 April 2001. We thank S. Ginell and A. Joachimiak (APS) and M. MacDonald and E. Duke (Daresbury) for their help and advice with synchrotron data collection; P. Nissen for advice on making Fig. 3; and B. T. Wimberly and K. Nagai for comments on the manuscript. Supported by the Medical Research Council (U.K.) and grant GM 44973 from the NIH to S. W. White and V.R.. D.E.B. was the recipient of a Human Frontier Science Program postdoctoral fellowship.
Funders:
Funding AgencyGrant Number
Medical Research Council (UK)UNSPECIFIED
NIHGM44973
Human Frontier Science ProgramUNSPECIFIED
Issue or Number:5518
Record Number:CaltechAUTHORS:20181030-134119461
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20181030-134119461
Official Citation:Recognition of Cognate Transfer RNA by the 30S Ribosomal Subunit BY JAMES M. OGLE, DITLEV E. BRODERSEN, WILLIAM M. CLEMONS JR., MICHAEL J. TARRY, ANDREW P. CARTER, V. RAMAKRISHNAN Science 04 May 2001: Vol. 292, Issue 5518, pp. 897-902 DOI: 10.1126/science.1060612
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:90512
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:30 Oct 2018 23:08
Last Modified:03 Oct 2019 20:26

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