Supplemental Figure 1a                                                                                         

MJs61a    ------------------------------------------------------------
MVs61a    ------------------------------------------------------------
PHs61a    ------------------------------------------------------------
MetBs61a  ------------------------------------------------------------
MKs61a    ----------------------------------------------------------MA
MTs61a    -----------------------------------------------------------M
FAs61a    MTEIRRNKGAAIPVIFIFALFIVVFYYFSHYTGLKLFILGLLSSPLFIIAYLLLSYKGPK
ThAs61a   -----------------------------------------MTEAGRNNSVMVASYKGPK
Hspsec61a ------------------------------------------------------------
AFs61a    -----------------------------------------------------------M
PyAs61a   ------------------------------------------------------------
APs61a    -----------------------MIQRVPLCQRGDTQFLTSDSNTGFPGISWFQLGGCIH
SAs61a    ------------------------------------------------------------
HSsec61a  ----------------------------------------------------------MA
SCsec61a  ---------------------------------------------------------MSS
BSsecy    ------------------------------------------------------------
StrCsecy  ------------------------------------------------------------
AAsecy    ------------------------------------------------------------
ChTsecY   ------------------------------------------------------------
CTsecy    ------------------------------------------------------------
Syspsecy  ------------------------------------------------------------
TTsecY    ------------------------------------------------------------
FNsecY    ------------------------------------------------------------
LIsecy    ------------------------------------------------------------
TMsecy    ------------------------------------------------------------
ECsecy    ------------------------------------------------------------

                             >-------------1--------------<
                 HHHHHHH           HHHHHHHHHHHHHHHHHHHHH              
                                                      a                               
          ---••••,••••1••••,••••2••••,••-••3••••,••••4••••,••••5••---- 
MJs61a    ---MKKLIPILEKIPEVELPVKEITFKEKL-KWTGIVLVLYFIMGCIDVYTAGAQI----
MVs61a    ----MKIKPILELIPEVKRPLKGVSFKEKI-QWTGLVLILYFILGTIDIYMGGAEM----
PHs61a    MGARDVIYAMEKWFPEVERPKKHVPLKEKF-VWTGLALVLYYVLAEIPVYGIPKKI----
MetBs61a  MTLRDTLEPFFNKLPAVASPEKHVHFKDKL-WWTLGVLLLYFALANVPLFGMSQDS----
MKs61a    DDWLERLRPILERLPEVKVPDRHVPFNEKLFKYTGIPLILYFILCEIPLYGLSPQA----
MTs61a    EQLKEKFEPLFSVLPQVKSPGYRVPFREKL-KWTGIILVLYFFLAQIPLYGLSANA----
FAs61a    KSRLYGLENLTAKLPAIKKAKGHVPFKYKL-MWTAVVVLIYFALTNIYIYGLNTSKT---
ThAs61a   KSKLYGLEDLTAKLPAVKKAKGHIEFKYKM-MWTALIVVLYFALSNIYIYGLNTAQT---
Hspsec61a MGWKEAAAPVLTRMPAVERPEGHVPFRRKM-YWTGGVLVLYFFLTNVPLWGIQTAG----
AFs61a    DSVIRALQPYFERIPSVERPKGHVHFREKF-GWTAAILLLYFILSNVPVFGLSPES----
PyAs61a   ------MMNFLTLIPTVSRPPRRLPLSKRL-FWTAVVAIVYIMMTITPLYGVQHTTQQG-
APs61a    LGVIDVLAAVGERFPAVRKPERKPTLYRRL-AWTGVILVLYFIMSNIPLYGIPPQN-I--
SAs61a    MGFMDFLAKMGENLPAVSKPKDKPTLTRKL-LWTFIGLIVYLLMASIPLYGVTSSNSF--
HSsec61a  IKFLEVIKPFCVILPEIQKPERKIQFKEKV-LWTAITLFIFLVCCQIPLFGIMSSDS---
SCsec61a  NRVLDLFKPFESFLPEVIAPERKVPYNQKL-IWTGVSLLIFLILGQIPLYGIVSSET---
BSsecy    --------------------MRVSDIRNKI-IFTLLMLIVFRIGAFIPVPYVNAEAL---
StrCsecy  ------------MLTAFARAFKTPDLRKKL-LFTLGIIVVYRLGTHIPIPGVDYKNVQEC
AAsecy    ------------MSEYLKALFELKELRQKF-IFTLLMFVIYRLGSHIPIPGINPEALR--
ChTsecY   ----------MKLTESIRNINKIPELRQRI-LYTLLLLFVYRLGSHITIPGVDASAVAS-
CTsecy    -------------MATLRQVFSISELRQKI-FFTFSLLALCRIGVFIPVPGINGDRAVAY
Syspsecy  --MVVSRDKAPSAQETFLQMAQAAGLRGRL-LITIGLLILVRVGIFIPVPDIDRQAFS--
TTsecY    ------------MLKAFWSALQIPELRQRV-LFTLLVLAAYRLGAFIPTPGVDLDKIQEF
FNsecY    ------MTLMEKFNSRLSSIVKIPELRERI-IFTLLMFLVARVGTLIPAPGVDVDRLS--
LIsecy    ------------MLTTFKNIFRIPELRQKI-IFTLSMLLLFRMGTHITIPGINPVVVAG-
TMsecy    ------------MWQAFKNAFKIPELRDRI-IFTFLALIVFRMGIYIPVPGLNLEAWGEI
ECsecy    -------MAKQPGLDFQSAKGGLGELKRRL-LFVIGALIVFRIGSFIPIPGIDAAVLAKL
                 ••••,••••1••••,••••2•••-•,••••3••••,••••4••••,••••5••

Supplemental Figure 1a (cont)
                      >----2a---------|------------2b--------------<
                        HHHHHHH              HHH HHHHHHHHHHHH
                          aa aaa a          °   °a 
          ----------••,••••6••••,••--••7••••,••••8••••,••••9---••••,••
MJs61a    ----------PAIFEFWQTITASRI--GTLITLGIGPIVTAGIIMQLLVG---SGIIQMD
MVs61a    ----------PAMFAFWQTVTASKM--GTLITLGIGPIVTAGIIMQLLVG---SELISLD
PHs61a    ----------QDYFQFLRVVLAGRN--GSILTLGIGPIVTAGIILQLLVG---SELIRLD
MetBs61a  ----------VDLFESYRAFFAGAS--GSLILLGIGPIVTASIVLQLLVG---ADIIKLD
MKs61a    ----------VDYFANLRAVLAGNF--GSILTLGIGPIVTASILLQLLVG---GDLIKLD
MTs61a    ----------VDQFAQFRAVLAGNF--GSILTLGIGPIVSASIILQLLVG---GKILKLD
FAs61a    ----------VDVFASFRAIFAGAS--GSLMDLGIGPIVTASIVMQLFAG---AKIFNLD
ThAs61a   ----------IDVFASFREIFAGAS--GSLMDLGIGPIVTASIVMQLFVG---AKIFNLD
Hspsec61a ----------NDFFGQFRSLLAGGQ--GTVLQLGIGPIVTASIVLQLLGG---ANLLGLD
AFs61a    ----------IDIFAAYRALFAGST--GSIIALGIGPIVTASIILQLLVG---AGIIKLD
PyAs61a   ---------TQPLQQLLSIIFGTAY--GTLAHLGIGPIVIAGILLEVFAF---SGILNLD
APs61a    ----------GGQVDLQRIIFASSA--GTLMELGIGPIVTASLIIQVLVG---AKIIKLD
SAs61a    ----------LSNFLAQQIIFASSQ--GTLAQLGIGPVITSGLIMQILVG---SKLINVD
HSsec61a  ----------ADPFYWMRVILASNR--GTLMELGISPIVTSGLIMQLLAG---AKIIEVG
SCsec61a  ----------SDPLYWLRAMLASNR--GTLLELGVSPIITSSMIFQFLQG---TQLLQIR
BSsecy    ---QAQSQ--MGVFDLLNTFGGGALYQFSIFAMGITPYITASIIIQLLQMDVVPKFTEWS
StrCsecy  V-DQASGN--QGLFGLVNMFSGGALLQITFFALGIMPYITASIILQLLTVVI-PRLEALK
AAsecy    D-FLKAFE--GSVFALYDIFSGGNLGRLTVFALGVMPYISASIMMQLLTVAI-PSLQRLA
ChTsecY   --ATTSHS--NDLFGLFDLFVGGAFARASIFSLGIMPYISASIIIQLLGAVT-PYVQKLQ
CTsecy    FNQLLGSS--RGLFQLADIFSGGAFAQMTVIALGVVPYISASIIVQLLVVFM-PTLQREM
Syspsecy  ---QAIND--NSVIGFLNIFTGGGLSTVGIFALGILPYINASIIMQLLTAAI-PALEDLQ
TTsecY    L-R--TAQ--GGVFGIINLFSGGNFERFSIFALGIMPYITAAIIMQILVTVV-PALEKLS
FNsecY    ---SMASQ--SDVLSYINMFSGGAFTRISIFSLGIIPYINASIVVSLLVS-IIPQLEEIQ
LIsecy    -IANDPSS--EGLLGMVDLFAGGALLKFSIFALGIMPYISSSIVMQLFMVLV-PALQKLQ
TMsecy    FRRIAETAGVAGILSFYDVFTGGALSRFSVFTMSVTPYITASIILQLLASVM-PSLKEML
ECsecy    L-EQ--QR--GTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVH-PTLAEIK
          •-•,--••--••60•••,••••70•••,••••80•••,••••90•••,••••-10•••,•

                  >-----------3-------------<
               HHHHHHHHHHHHHHHHHHHHHHHHHH
                                      
          ••-10•••,••••11•••,••••12•••,••-••13----••••,••-------------
MJs61a    LS-IPENRALFQGCQKLLSIIMCFVEAVLFV-GAG-----AFGILTP-------------
MVs61a    LS-KPMNRALFQGLQKLFGIFLCFLEAVMFV-GAG-----AFGVVNS-------------
PHs61a    LA-NPEDRRFYQALQRVFSVFMCFFEAAIWV-LGG-----AFGRVGVD----------VT
MetBs61a  LS-DPKDQSFFQGAQKFLVFVMIILEALPQL-LGG-YIQPDPGLASSL-----GV---GL
MKs61a    LT-NPEDRRLFQGLQKLLAIVLCFFEGVMMV-FSG-----AAPPAEPS----------I-
MTs61a    LS-RHEDKAFFQGLQKLLAIVFTFFEALIFV-LTG-----SLAPSAP-------------
FAs61a    LS-DSSDKAIYQGFQKLLVIIMIFVEAIPQA-FG--FLVPDAGLVSNIAHVVPGY---GE
ThAs61a   LQ-NAEDKAIYQGVQKLLVIIMIFVEAIPQA-FG--YLVPDTKVVNSINAVFPGY---GE
Hspsec61a TDNDPRDQAIYQGLQKFLVGVMVVLTGAPMV-FLGNFLQPSQQLAQSM-----PG---GA
AFs61a    LT-NPEDRAAYQDFQRFLVFVMIAVEAIPQI-AGG-LLKPDLNLAAQL-----GV---SP
PyAs61a   LN-KREDRLKFTLLLKWTALGIAAVEAVAYV-LSG-----QFGPVTP-------------
APs61a    LA-DPEGRRKFTSAQKVLALAFAALEAVAFT-VGG--RYWVGTAIEPG------------
SAs61a    LT-TQEGKSKFTQAEKALALIFIIVESSLF---G-----YVFTRATSN------------
HSsec61a  -D-TPKDRALFNGAQKLFGMIITIGQSIVYV-MTG-----MYGDPSEMG-----------
SCsec61a  PE-SKQDRELFQIAQKVCAIILILGQALVVV-MTG-----NYGAPSDLG-----------
BSsecy    KQ-GEVGRRKLAQFTRYFTIVLGFIQALGMS-YGFN--NLANGMLIEKS----------G
StrCsecy  KE-GQAGTPKITQYTRYLTVALAILQGTGLV-ATARSGALFSGCTVAGQIVPDQA-----
AAsecy    KEEGDYGRYKINEYTKYLTLFVATVQSLGIA-FWIRGQVSPKGIPVVENP---------G
ChTsecY   KE-GEEGRQKINQYTRYGTILIALLQAWGVS-VNLSSPSSF-GKVVVPD--PGFF-----
CTsecy    RESPDQGKRKLGRMTRLFTLVLACVQSLLFAKFALRMNLVVPGIVLPAMLSLKLFG--VP
Syspsecy  KNEGEAGRRKISQYSRYIAFGWCIIQGLGLT-VG-----LLRPYANNYG-----------
TTsecY    KE-GEEGRRIINQYTRIGGIALGAFQGFFLA-TAFLGAEGGRFLLPGWSP---------G
FNsecY    KE-GESGRNRITQWTRYLTIALAIIQGTGVCLWLQ-----SVGLIYNPG-----------
LIsecy    KE-GEEGRKKIGQYTKYGTVILCAIQSLAV--IQLAKGWST-GTELEPARYPGLINSSVV
TMsecy    RE-GEEGRKKFAKYTRRLTLLIGGFQAFFVS-FS-LARSNPDMVAPGVN----------V
ECsecy    KE-GESGRRKISQYTRYGTLVLAIFQSIGIA-TG--LPNMPGMQGLVINP---------G
          ••-•11•••,••••12•••,••••13•••,•-••--•14•••,••••15•---------•   

Supplemental Figure 1a (cont)
           >-----------4------------<      >-----------5--------------
           HHHHHHHHHHHHHHHHHHHHHHHHH       HHHHHHHHHHHHHHHHHHH 
                                            å   å  
          -••14•••,••••15•••,••••16••-•,••••17•••,••••18•••,•••-------
MJs61a    -LLAFLVIIQIAFGSIILIYLDEIVSK-YGIGSGIGLFIAAGVSQTIFVGALG-------
MVs61a    -TLALILVLQLALGAILVIYLDEIVSR-YGIGSGIGLFIAAGVAQTIFVGAFG-------
PHs61a    YTIATLMIIQLALGGIILIVLDELVSK-WGIGSGISLFIAAGVSQRILTRSLNPLTDPNI
MetBs61a  GVITFLLLIQIFIGGALILFMDEVVSK-WGIGSGVGLFIVAGISQQIVTGIFNWQLD---
MKs61a    -LLEILLILQLALGGILVIFLDEVVSK-WGIGSGVGLFIVAGVSSQIIIGAFNPLPSP--
MTs61a    -QFVWVLILQLTIGGILIIFLDEVVSK-WGFGSGVGLFIAAGVSQEIIVGAFNPLSAP--
FAs61a    FLAQSIIILQLFFGSYLVFLMDEVVSK-YGIGSGISLFIAAGVSQQLFTGTFNWIPSTIT
ThAs61a   FLAQTIIILQLFFGSYLVFLMDEVVSK-YGIGSGISLFIAADVSEQLFIGAFNWQALNNA
Hspsec61a FGVEVLIFAQIAAGGILLLFMDEVISK-WGVGSGIGLFIVAGVSQSLVGGLVFWEG----
AFs61a    GIISFLIFIQLFIGGVLIVYMDEVVSK-WGIGSGVSLFILAGIAQSIVVGLFNWVIPP--
PyAs61a   -LGGLLIVLQLLIATVIIILLDDLMSKGWGIGSAISLIIFLSVSKQIFLSLFSWDVAVDN
APs61a    PLDYALVSLQLFLGALLVIYFDEVMQKGWGIGSAISLFILAGVAQGVVWSIFG-------
SAs61a    ILLPIIVVVQLIIASYIILLLDEMIQKGWGLGSGVSLFIMAGIMKVIFWNMFGIVSVQ--
HSsec61a  AGICLLITIQLFVAGLIVLLLDELLQKGYGLGSGISLFIATNICETIVWKAFSPTTVNT-
SCsec61a  LPICLLLIFQLMFASLIVMLLDELLSKGYGLGSGISLFTATNIAEQIFWRAFAPTTVNS-
 BSsecy    VSTYLIIALVLTGGTAFLMWLGEQITS-HGVGNGISIIIFAGIVSSIPKTIGQ-------
StrCsecy  IFTTVVMVICMTAGTCVVMWLGELITD-RGIGNGMSILMFISIAATFPSALWA-------
AAsecy    ISFILITVLTLVAGTMFLVWIADRITE-KGIGNGASLIIFAGIVANFPNAVIQ-------
ChTsecY   --FTITAVIILTASTVFVMWLGELITE-RGIGNGISLIIMIGILARFPASLVA-------
CTsecy    WVFYLTTVVVMTTGTLLLMWVGEQISD-KGIGNGISLIITLGILASFPSVLGS-------
Syspsecy  PLFIFQTVLAITAGSMFVMWISELITE-RGIGNGASLLIFVNIVATLPQTLGQ-------
TTsecY    PFFWFVVVVTQVAGIALLLWMAERITE-YGIGNGTSLIIFAGIVVEWLPQILR-------
FNsecY    ISFFVRTITTLTAGTVFLMWVGEQISI-KGIGNGVSLIIFLNVISRAPSSVIQ-------
LIsecy    PYFYLIGILSITTGTVLLIWLGEQITE-RGIGNGISLLIFAGIIGRLPESMVQ-------
TMsecy    LQFTVLSTMSMLAGTMFLLWLGERITE-KGIGNGISILIFAGIVARYPSYIRQ-------
ECsecy    FAFYFTAVVSLVTGTMFLMWLGEQITE-RGIGNGISIIIFAGIVAGLPPAIAH-------
          •,••••16•••,••••17•••,••••18•••,•••••19•••,••••20•••,-------

          ---------------------<                              >-------
                        HHHHHHHH                                 HHHHH 

          ---------•19•••,••••20••---•,••---------------------••21•••,
MJs61a    ---------PEGYLWKFLNSLIQG---VPNI---------------------EYIAPIIG
MVs61a    ---------AEGYLWKFFSAMSVG---SLGIA-F------------------EYILPILS
PHs61a    IDPLTGKPAIVGAIPYFIQHILDGD--LKGALYRGGS--------------APDMIAVTA
MetBs61a  ----SSG-LPVGLIPKWIYIAQNVG--ADYLLSGEGVLYMLVS--------GG-ILALLS
MKs61a    ---QQPG-RPAGAVWAFLYSAMQG---TPDWT---------------------LLAPVIG
MTs61a    ---TQPG-VPAGRITGFLYLLFTGQ--SPDFQY--------------------YVLPVLA
FAs61a    SPLSLSN-PPAGAIPKALYLFINAP--GSYLTNTGMEQILFAQ--------PNPMIALVG
ThAs61a   LTYSLTN-PPAGAFPKMFYIIMHSS--SSYLLTNGVVQILFSP--------PNPMIAVLG
Hspsec61a -----GV-GSQGLLPTWFDIIVGNVSNMPPLLSGSGIEFLLMQ--------AG-ILGLLT
AFs61a    ----NSA-MPAGIIPRWIWIAQNYP--LDQLFTGSGLAFLLIQ--------GG-ILALIT
PyAs61a   ----ADQRHIVGLIPALGAALYDLFAHGNAASIMGLINREVWLKGQQTPTYLPDFIGLIS
APs61a    --------TIPGVAQDYGLVPAIIS--NPDLTLLARPNG------------FPDLTGFFT
SAs61a    -----SQNLPVGFFPLLVSYITSGRNLQEIVLNTSSTTP-----------YQPDLIGLIA
HSsec61a  ---GRGM-EFEGAIIALFHLLATRT--DKVRALREAFYRQN----------LPNLMNLIA
SCsec61a  ---GRGK-EFEGAVIAFFHLLAVRK--DKKRALVEAFYRTN----------LPNMFQVLM
BSsecy    ----------------IYETQFVGS--NDQLFIH----------------IVKVALLVIA
StrCsecy  -------------------IKKQGE-LADGWI--------------------EFGTVILV
AAsecy    ----------------FYEKVKTG----DIGP-------------------LTLLLIIAL
ChTsecY   ----------------EAQSVSFGS--KNWIV---------------------EIVIMAL
CTsecy    ----------------IFNKLNLGSQDPSEFG------------------IVSLLILCAV
Syspsecy  ----------------TIEYAQSGG--RQSIT--------------------AVVLLMLV
TTsecY    ----------------TIGLIRTGE--VNLVA---------------------FLFFLAF
FNsecY    -----------------TIQTMQGN--KFLIP--------------------LLVLVAFL
LIsecy    -------------------LFSTDT--MDALN---------------------VLILLIL
TMsecy    --------------------AYLGG--LNLLE---------------------WIFLIAV
ECsecy    ----------------TIEQARQGD--LHFLV---------------------LLLVAVL
          ----------------••••21•••--,••••---------------------22•••,•

Supplemental Figure 1a (cont)
          --6------------<                              >-----------7-
          HHHHHHHHHHHHH SSSSSSS              SSSSSSS      HHHHHHHHHHHH 
                                                         a  a å  a   a
          ••••22•••,••••23•••,••••-------24•••,••••25•••,••••26•••,•••
MJs61a    TIIVFLMVVYAECMRVEIPLAHGR-------IKGAVGKYPIKFVYVSNIPVILAAALFAN
MVs61a    TLFVFLVVVYVESIRVEIPLAHGR-------VKGAVGKYPIKFIYVSNLPVILAAALFAN
PHs61a    TIIVFLVVVYFESMRVEIPLGYRG-------VT-IRGRYPIKFLYVSNIPIILTFALYAN
MetBs61a  TIVIFLLVVYVESTRIEIPLAHSA-------VRGARGRFPVKLIYASVLPMILVRALQAN
MKs61a    AIITFLIVLYVEGMRVEIPIAFAG-------IRGARGRFPVRLLYTSNIPVILASALFMN
MTs61a    LIAVFLVVVYAESMRVEIPISMGGG---KRLSRGAVGKYPLRFIYASNMPVILTSALLLN
FAs61a    TLLIFFVVAFFQSSKIELPIAHER-------VRGARGRYPLQLLYASNIPVILATALLAN
ThAs61a   TLLIFFLVAYFQSSKIELPISHER-------VRGARGRYPLQLLYASNIPVILATALLAN
Hspsec61a TLFIYVVVVYAESVRVEIPLSHAR-------VKGARGRFPVKLIYASVLPMILVRALQAN
AFs61a    TAAIILLVVFFEGTRVEIPLAHAV-------ARGARGRFPIKLIYASVLPMIFVRALQAN
PyAs61a   TLLLAYIILYLEMMKVNIPVTAGQ-------YRGIKFTIPLRFVYVSVLPIIFTTYSLLL
APs61a    TLAAIILLVYLQAMRVEIPITSER-------FKGIRSRVPLQFIYVTNIPILLVGILVSD
SAs61a    TVGLTILIVYLVNTNIYIPVTTQR-------LRGIRTTVPLNFLYVSSIPVIFVSVLGAD
HSsec61a  TIFVFAVVIYFQGFRVDLPIKSAR-------YRGQYNTYPIKLFYTSNIPIILQSALVSN
SCsec61a  TVAIFLFVLYLQGFRYELPIRSTK-------VRGQIGIYPIKLFYTSNTPIMLQSALTSN
BSsecy    ILAVIVGVIFIQQAVRKIAIQYAKGTGRSPAGGGQSTHLPLKVNPAGVIPVIFAVAFLIT
StrCsecy  GLVMVGLVVFVEQAQRRIPVQYAKRMIGRRSYGGTSTYIPLKVNQAGVIPVIFASSLLYI
AAsecy    IIAIIVGIVYVQEAERRIPIQYPGRQVGRQLYAGRKTYLPIKINPAGVIPIIFAQALLLI
ChTsecY   MVAIVAIVVILTVATRRIPVQHAKRVVGRKVYGGGTQYIPIKINTAGVMPIIFAQSIMFL
CTsecy    FVFVLIATVLIIEGVRKIPVQHARRIIGRREVVGGGSYLPLKVNYAGVIPVIFASSLLMF
Syspsecy  FLVMIVGIVFVQEGTRRIPIISARRQVGKKLYRERTSYLPLRLNQGGVMPIIFASAVLIL
TTsecY    IVLAFAGMAAVQQAERRIPVQYARKVVGRRVYGGQATYIPIKLNAAGVIPIIFAAAILQI
FNsecY    GTVTIAGIVLFQLGQRKIPIHYVGKGFSSKGGIGEKSFIPLRLNTAGVMPVIFASVFMLI
LIsecy    FILLISLTVLLTQGVRKVPLQYGKQMVGRKMVQAKSQSIPFKVNGANVMPIIFASSLILF
TMsecy    ALITIFGIILVQQAERRITIQYARRVTGRRVYGGASTYLPIKVNQGGVIPIIFASAIVSI
ECsecy    VFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIPAIFASSIILF 
          •••23•••,••••24•••,••••25•••,••••26•••,••••27•••,••••28•••,•
          
          ---------<
          HHHHHHHH                   SSS            SSS   HHHH  

          •27•••,•••----------•28•••,•••----------•29•••,-••••30•••,--
MJs61a    IQLWGLALYR----------MGIPILGHYE----------GGRAVDG-IAYYLSTPYG--
MVs61a    IQLWGMFLDR----------MGYPILGQYS----------NGTAVSG-IAYYFSTPYG--
PHs61a    IQLWARVLDR----------FGHPWLGRFDPV--------TGNPIGG-FVLYVIPPRN--
MetBs61a  IQMIGIILA----------SRGITFFGEFH----------GSTPLNG-IMYYLAPIHSPY
MKs61a    VRLWALAFQR----------MGVPILGKLDP---------RGQPISG-LVYYLSPPNS--
MTs61a    VQLLANVFQK----------LGYPILGTVS----------NGQAVDG-LAYLLTAPRS--
FAs61a    ISMWTLLFWDSPVLSKIPILGHSHLLGSYASAAQISALGISSTTPTGGLAYYLYTPNGLS
ThAs61a   VSMWTLLFWSSPVLSKVPILGHNPLLGSYPTTAQATALNISATTPTGGLAYYLFSPNGLS
Hspsec61a IQFLGQILNST-------LASMPTWLGVYG----------GNGQVTGGLFYYLAPIYSPN
AFs61a    VVALGQVLH----------ARGVTIFGEFV----------NGKAVSG-LMFFLQPVSSPY
PyAs61a   VGQLLSPFQQNP-------------------------------VIEAILMIIFPLHRD--
APs61a    LLLVQRLLAD--------------YLGVESR---------AYQIYSSIV-YYLSPPRGVV
SAs61a    IQLFASLAN-----------------SISNS---------ASGILTDIANAFFFPPQGVP
HSsec61a  LYVISQMLSARFS-----GNLLVSLLGTWSD--TSSGGPARAYPVGG-LCYYLSPPES--
SCsec61a  IFLISQILFQKYP-----TNPLIRLIGVWG---IRPGTQGPQMALSG-LAYYIQPLMS--
BSsecy    PRTIASFFGTN--------------------------------DVTKWIQNNFDN-----
StrCsecy  PALIVQFSNSTAG-----------------------------WATWI-TKNLAD------
AAsecy    PSTLLNFVQN-P-------------------------------FIKV-IADMFQ------
ChTsecY   PSTFLSFFPESE-------------------------------AMQK-VAAAFA------
CTsecy    PATIGQFLSSES------------------------------SWLKR-IATMLS------
Syspsecy  PSSLAGFATGNE--------------GLG-----------GFGEIFVQISNALR------
TTsecY    PIFLAAPFQDNP-------------------------------VLQG-IANFFN------
FNsecY    PGVIVNALPST-------------------------------LSIKTTLSIIFG------
LIsecy    PQTIIQWLSNSSQ----------------------------EWAGWA-VIMDFFNPFS--
TMsecy    PSAIAS-ITNNE-------------------------------TLK----NLFR------
ECsecy    PATIASWFGGGTG----------------------------WNWLTT-ISLYLQ------
          •••29•••,••••----------------------------30•••,-••••31------

Supplemental Figure 1a (cont)
                                   >---------8----------<
                                   HHHHHHHHHHHHHHHHHHHHHHH      HHHHHH           
                                                         
          --••••31••---------------•,••••32•••,••••33•••,••••34•••,•••
MJs61a    --LSSVISDP---------------IHAIVYMIAMIITCVMFGIFWVETTGLDPKSMAKR
MVs61a    --ISNIISDP---------------LHAIFYTLMMVIFCILFGLFWVETSGLDAKSMAKK
PHs61a    --IFTVIDNP---------------VRAIIYLILTIIFSLLFGFLWVELTGLDARTIARQ
MetBs61a  DWIPSLVRQSFSGYGAATPANWQIVLHVFTDATMLVVGGIIFALFWIETTGMGAKPTAQK
MKs61a    --IVKTLSDP---------------LQALGYMMAMVIASVFFAVLWVELTGMGPREIARH
MTs61a    --IDALILDP---------------FRVVFYAVVFIGLCVLFAWLWVEISNIGPRHVARQ
FAs61a    DWLFPILQPSVSQSVLLGHTPVEELIHIVVFMVFLVGFSILFAKFWIETTNMGPDAVAKQ
ThAs61a   DWLFPILQPSGYQNILLGHTPLQEAIHIIVFTAFMVGFSVLFAIFWIETTNMGASAVAKQ
Hspsec61a AWMWWTSGA----T----AARWQVLIRIAIDLSFMIIGGAIFAIFWVETADMGPDATARQ
AFs61a    DWIPSLVKS--QGAAFAAIPDWMIYLHLLIDALILVVGGIIFAWFWVETSGMDARTVASQ
PyAs61a   ------FSN---------------VPALVLHYLIYVALAIAFAWVWVQLAGLSAEDQARQ
APs61a    QS----IADP---------------VKTAVFIASWTVLSIVFGYMWVEIAGLNPREQAER
SAs61a    HSVYALVVDP---------------VGAAIYAAVFIVLSIVFGMLWIDVAGLDPKTQAEQ
HSsec61a  --FGSVLEDP---------------VHAVVYIVFMLGSCAFFSKTWIEVSGSSAKDVAKQ
SCsec61a  --LSEALLDP---------------IKTIVYITFVLGSCAVFSKTWIEISGTSPRDIAKQ
BSsecy    --------------------------THPVGMAIYVALIIAFTYFYAFVQ-VNPEQMADN
StrCsecy  -------------------------TAATPHIILYFFLIVFFAFFYVAIS-FNPEEVADN
AAsecy    -------------------------PGAIFYNFLYVTFIVFFTYFYTAVL-INPVELAEN
ChTsecY   -------------------------YDSWFYAVVFGLMIVFFTYFYTAIA-FNPKEVADT
CTsecy    -------------------------PGSVAYSIFYVLLIIFFTYFWTATQ-FRPEQIASE
Syspsecy  -------------------------PGTWVYTVVYSVMIFFFSYFYASLI-VNPEDVSKN
TTsecY    -------------------------PTRPSGLFIEVLLVILFTYVYTAVQ-FDPKRIAES
FNsecY    -------------------------QNHPVYMILYALVIMFFSFFYTALV-FDPEKVAEN
LIsecy    -------QIW---------------YHALFYFVIYTALIVFFAYFYTAIQ-FNPAELAEN
TMsecy    -------------------------AGGFLYLLIYGLLVFFFTYFYSVVI-FDPREISEN
ECsecy    -------------------------PGQPLYVLLYASAIIFFCFFYTALV-FNPRETADN
          -------------------------•••,••••32•••,••••33•••,•-•••34•••,

                                         >---------9------------------
          HHH               HHHHHHHH  HHHHHHHHHHHHHHHHHHHHHH                

          •35•••,••••36••---•,••••37•••,••••38•••,••••39•••,••••------
MJs61a    IGSLGMAIKGFRKSE---KAIEHRLKRYIPPLTVMSSAFVGFLATIANFIGALG------
MVs61a    LGNLDMAIKGFRKSQ---KSIEQRLKRYIKPITVMGSAFVGFLAAAADFTGALG------
PHs61a    LQRAGLQIPGFRRDP---RTLERVLQKYIPYVTFWGSLTVALISVLADFLGALG------
MetBs61a  IFNSGMQIPGFRRNI---SSIEKVTQRYIPKVTVIGGAFIGLLTLIASLLGTLGS-----
MKs61a    LHRAGLHIPGFRRDI---RVLEKRLQKYIYPVTVMGGAFVGFLAAGADLMGALG------
MTs61a    LYQMGMQIPGFRSSR---GQFEKILKRYIPTITILGGAFVGLLAFVADLTGSLG------
FAs61a    IRSSGMQIPGFRRDP---RIMAKVLSKYIPAITIFSGAIVGILAGAADLIGTVGD-----
ThAs61a   IQASGMQIPGFRRDP---RVMERVLKKYIPAITIFSGAVVGLLAAGANLIGTVGD-----
Hspsec61a IQNSGMQIPGFRKNQ---GVIEKVMERYIPQVTVIGGALVGLLAVMANMLGTIGN-----
AFs61a    IAKSGMQVPGFRKSP---QVLERVLSRYIPKVTILGGAIIGILTLVANMLGTIGN-----
PyAs61a   FARSQLHVPGFRQSE---KILAKILERPINALTIISGFIAGSFAALGNILGVWG------
APs61a    LIKGGLAIPGMRSDP---RVLERVLRRYIYPLTFLSSLIVAALVIVADIFGAYG------
SAs61a    MIRSGIEIPGMRTNP---RIIEGILSKYIYALGFFSSLIVGLIAVVATFLGTYG------
HSsec61a  LKEQQMVMRGHRET-----SMVHELNRYIPTAAAFGGLCIGALSVLADFLGAIG------
SCsec61a  FKDQGMVINGKRET-----SIYRELKKIIPTAAAFGGATIGALSVGSDLLGTLG------
BSsecy    LKKQGGYIPGVRPGKMTQDRITSILYRLTFVGSIFLAVISILPIFFIQFAGLPQS-----
StrCsecy  MKKYGGFIPGIRAGRPTAEYLSYVLNRITWPGSLYLGLIALVPTMALAGFGANQN-----
AAsecy    LHKAGAFIPGVRPGQDTVKYLERIINRLIFFGALFLSVIALIPILISVWFNIP-------
ChTsecY   MRRQGGFIPGVRPGKNTEEFIDNILTRITLPGAIALAVIAILPTFLTKFVNVTPG-----
CTsecy    MKKNGAFIPGIRQGKPTQTYLEYTMNRVTLLGAVFLAVVAILPSVLGRILRVDAN-----
Syspsecy  LKKMGSSIPGIRPGKKTEQYLEGVLNRLTFLGAIFLSFVATLPIFVEQATGVTTFQG---
TTsecY    LREYGGFIPGIRPGEPTVKFLEHIVSRLTLWGALFLGLVTLLPQIIQNLTGIHS------
FNsecY    LKQGGGTIPGIRPGEETVEYLEGVASRITWGGGIFLAIISILPYVIFTSMGLPVY-----
LIsecy    LKKYGGFIPGIRPGSHTKEYIEKVLNRITLPGAMFLAGLALAPYIIIKFLDLSSNSGGGS
TMsecy    IRKYGGYIPGLRPGRSTEQYLHRVLNRVTFIGAVFLVVIALLPYLVQGAIKVN-------
ECsecy    LKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMRDAMKVP-------
          ••••35•••,••••36•••,••••37•••,••••38•••,••••39•••,•••-------

Supplemental Figure 1a (cont)
          ------------10------<
                HHHHHHHHHHHHHHHHHHHHHH  HHH
                    aå  a            y  y
          -----40•••,••••41•••,••••42•--••,••••43•••,•----
MJs61a    -----GGTGVLLTVSIVYRMYEQLLREK--VSELHPAIAKLLNK----
MVs61a    -----GGTGVLLTVSIVYRLYEQLVQEQ--LSELHPAVAKFVGKR---
PHs61a    -----TGTGILLTVGILYRFYEEIAREQ--ITEMFPALRRLFKG----
MetBs61a  ----TSGTGLLLAVSIVYRLYEDIASEQ--MMEMHPMIRSFFGEQ---
MKs61a    -----GGTGVLLTVSILYNMYEEIKQER--LMEAHPVVRKFLEKTLR-
MTs61a    -----GGTGVLLTVGIVYRLYEEIAQEQ--LMDMHPILRSFLGD----
FAs61a    ----TSGTGLLLAVGIVIQFYEAMGREQ--LMEMHPLIRQFFNGG---
ThAs61a   ----TSGTGLLLAVGIIIQFYEAMGREQ--LMEMHPVIRQFFVGG---
Hspsec61a ----VSGTGLLLTISITYKLYEEIAEEQ--MMEMHPMMREMFGGGD--
AFs61a    ----VSGTGLLLAVSIAYRFYEDLAKEQ--LTEMHPLIRRMLGEEA--
PyAs61a   -----GGTGLILLVEIALQYYALVMREQ--MLEMYPGLRQILE-----
APs61a    -----TGTGLLLAVGIINQYYAMITRER--ALETYPLLRRILGEEVV-
SAs61a    -----TGVGLLLAITIAMQYYNLLAYER--TLEMYPLLKRIVGE----
HSsec61a  -----SGTGILLAVTIIYQYFEIFVKE----QSEVGSMGALLF-----
SCsec61a  -----SGASILMATTTIYGYYEAAAKEGGFTKNLVPGFSDLM------
BSsecy    --AQIGGTSLLIVVGVALETMKQLESQLV-KRNYRGFMKN--------
StrCsecy  --FPFGGTSILIIVGVGLETVKQIESQLQ-QRNYEGFLR---------
AAsecy    --FYFGGTTALIVVGVALDTFRQIETYLI-QKKYKSYVRR--------
ChTsecY   FANFFGGTSLLIVVSVGLDTLQQVESHLM-MRHYDGFMKTAKARGRR-
CTsecy    VSYFLGGTAMLIVVGVILDTMKQIDAFLL-VRRYDGVLKKDRPKGRP-
Syspsecy  ----LGATSLLILVGVAIDTAKQIQTYVI-SQRYEGMIKQP-------
TTsecY    --IAFSGIGLLIVVGVALDTLRQVESQLM-LRSYEGFLSRGRLRGRNR
FNsecY    ----FGGTGIIIVVGVALDTIQQIDAHLV-MRDYKGFI----------
LIsecy    LVYTFGGTSLLIMVGVALETLKQIESQLL-MRNYEGFMKKSKIKGRS-
TMsecy    --VWIGGTSALIAVGVALDIIQQMETHMV-MRHYEGFIKKGKIRGRR-
ECsecy    --FYFGGTSLLIVVVVIMDFMAQVQTLMM-SSQYESALKKANLKGYGR
          --•40•••,••••41•••,••••42•••,-••••43•••,••••44••

Supplemental Figure 1b

                                           •••-----•,••••1••••,••••-2
MJs61b   ----------------------------------MSK-----REETGLATSAGLIRYM-D
PHs61b   ----------------------------------MAK-----EKTTLPPTGAGLMRFF-D
MetBs61b ----------------------------------MAK-----KSGSGLQSSAGLMRYY-E
MKs61b   ----------------------------------MGKKME-KKRAEMPPARAGILSFW-D
MTs61b   ----------------------------------MAKK----DKKTLPPSGAGLVRYF-E
FAs61b   -------------------------------MTSMAKD----NQNENFQSGAGLIRYFNE
ThAs61b  ----------------------------------MASD----RKSEGFQSGAGLIRYFEE
Hsps61b  ----------------------------------MSSG----QNSGGLMSSAGLVRYFDS
AFs61b   ----------------------------------MAKAPKGKAKTPPLMSSAGIMRYF-E
PyAs61b  ----------------------------------MARRRK--YEGLNPFVAAGLIKFSEE
APs61b   -MGMRHSSQPYYGMLRFNSPHTHVTGGRVPGGWRVLSVRRRRERRATPVTAAGLLSFY-E
SAs61b   ----------------------------------MPSSKK-KKEDVPIASMAGLVRYY-E
HSsec61b MPGPTPSGTNVGSSGRSPSKAVAARAAGSTVRQRKNASCGTRSAGRTTSAGTGGMWRFYT
SCsbh1   ----------------MSSPTPPGGQRTLQKRKQGSSQKVAASAPKKNTNSNNSILKIYS

                  >---------TM-------<
                  HHHHHHHHHHHHHHHHHHHH
         ••••,••••3••••,••••4••••,••••5•••
MJs61b   ETFSKIRVKPEHVIGVTVAFVIIEAILTYGRFL-----
PHs61b   EDTRAIKITPKGAIALVLILIIFEILLHVVG-PRIFG-
MetBs61b ADKNAVQVQPKVVLIVGAIVGIAVLFLSAVN-GFWP--
MKs61b   EEAPGIKIDPDYILYACFAVAVLLIIAHTMAAV-----
MTs61b   EETKGPKLTPEQVVVMSIILAVFCLVLRFSG-------
FAs61b   EEIKGPAIDPKLIIYIGIAMGVIVELAKVFW-PV----
ThAs61b  EEIKGPALDPKLVVYMGIAVAIIVEIAKIFW-PP----
Hsps61b  EDSNALQIDPRSVVAVGAFFGLVVLLAQFFA-------
AFs61b   EEKTQIKVSPKTILAAGIVTGVLIIILNAYY-GLWP--
PyAs61b  GELEKIKLTPRAAVVISLAIIGLLIAINLLLPPL----
APs61b   EYEGKIKISPTIVVGAAILVSAVVAAAHIFLPAVP---
SAs61b   SEKEKVKISPKVVVVASIVLIAGVIIASFIIRPRYNLF
HSsec61b EDSPGLKVGPVPVLVMSLLFIASVFMLHIWGKYTRS--
SCsbh1   DEATGLRVDPLVVLFLAVGFIFSVVALHVISKVAGKLF

Supplemental Figure 1c

MJs61g   ------------------------------------------------------------
PHs61g   ------------------------------------------------------------
MetBs61g ---------------------------------------------------------MVE
MKs61g   ------------------------------------------------------------
MTs61g   ------------------------------------------------------------
FAs61g   -----------------------------------------------------MDKGIEG
ThAs61g  ------------------------------------------------MFCFGDLVSIED
AFs61g   ------------------------------------------------------------
PyAs61g  -----------------------------------MASPQEKLEKLWRDIRWVVSTASRP
APs61g   ------------------------------------------------------------
SAs61g   -----------------------------------------------------------M
Hsps61g  ------------------------------------------------------------
HSsec61g ------------------------------------------------------------
Scsss1   -----------------------------------------------MARASEKGEEKKQ
BSsecE   ------------------------------------------------------------
StrCsecE ------------------------------MTDAVGSIDMPDAQDEAPDSKKSRKGGKRG
AAsecE   ------------------------------------------------------------
ChTsecE  ------------------------------------------------------------
CTsecE   -------------------------------------------------MGQDHRRKFLK
SyspsecE ---------------------------------------------MVKKEAVRTDSTEDN
TTsecE   ------------------------------------------------------------
FNsecE   ------------------------------------------------------------
LIsecE   ------------------------------------------------------------
TMsecE   ------------------------------------------------------------
ECsecE   MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLRALAVVILIAAAGGVAL
         ••••,••••1••••,••••2••••,••••3••••,••••4••••,••••5••••,••••6

                                           >---------------TM--------
         HHHHHHHHHHHHHHHHHHHHHHHHHH    HHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
                                                       a  a         
         ••••,••••1••••,••••--2••••,••••3••••,••••4••••,••••5••••,•••
MJs61g   MKTDFNQKIEQLKEFIEEC--RRVWLVLKKPTKDEYLAVAKVTALGISLLGIIGYIIHVP
PHs61g   ----MPEFQERVRNFFKES--KRVFLVTKKPGWEEYKKAAKITGLGILVIGLVGMLIRII
MetBs61g STFEPKITAESVGQVIRAH--LRVLKLTRKPSREEFLTIAKVAGVGILAVGAIGFIIYVL
MKs61g   -----MSTYEELISLLRDC--KRVLRAARKPTWDEYIESAKIAGLGILIVGGVGFLIRVI
MTs61g   -----MKYRESILNFIKQS--KRVLRVSKKPSREEYLNVSKVTGIGIIIIGVIGFIISII
FAs61g   KLVEQQEKIERKFQGIGKGKYARILKMAKKPNGNEYTKVVLIAGSGIVLLGLIGFIIYYI
ThAs61g  KAEEIQQNLERRFSSIGKGKYARILRMARKPTRDEYIKVLVITAVGLLIIGGRWFHNIPA
AFs61g   -MDISGMQINEVQSKLKEY--YNVLKMARKPDWEEFSMTAKVALAVMFIVGFVGFVIYIL
PyAs61g  DDAEFKLAARFLLLLTFIAGAFQIVFHIAGLYLNSAVYRTEVTTLGDPVKETVATLASIV
APs61g   -----MGIVDIAREYIVAWR--RILTLARKPDEEEYSLLLKLNLLGFALVGGIGYLIHLG
SAs61g   KVFSIMKISDIIKRLREDW--KRIISVAKKPDKDSFNYSIRLTLLVMAVVGLIAYIVQLT
Hsps61g  --------MDVPLELSAYT---RVLRLASTPSWEEFSQIAKIAGAGILLIGAIGFLVFLI
HSsec61g -MDQVMQFVEPSRQFVKDS--IRLVKRCTKPDRKEFQKIAMATAIGFAIMGFIGFFVKLI
Scsss1   SNNQVEKLVEAPVEFVREG--TQFLAKCKKPDLKEYTKIVKAVGIGFIAVGIIGYAIKLI
BSsecE   -----MRIMKFFKDVGKEM--KKVSWPKGKELTRYTITVISTVIFFVIFFALLDTGISQL
StrCsecE KKGPLKRLALFYRQIVADV--RKVVWPSRNQLTTYTTVVIIFVVIMIGLVTLIDYGFSHA
AAsecE   ----MEKLKEFLKGVRDEL--KRVVWPSRELVVKATISVIIFSLAIGVYLWILDLTFTKI
ChTsecE  MKKYIEKVDKYYRDVVGEM--RKVSWPTREEVKDMTIVVLTVSGILALFTFVVDWVISTV
CTsecE   KVSFVKKQAAFAGNFIEEI--KKIEWVNKRDLKRYVKIVLMNIFGFGFSIYCVDLALRKS
SyspsecE SVDNVQARSNFIAATKDEL--AKVVWPSRQQLISESVAVILMVILVSTVIYFVDQIFGWI
TTsecE   ---MFARLIRYFQEARAEL--ARVTWPTREQVVEGTQAILLFTLAFMVYLGLYDTVFRFL
FNsecE   --------MNLFQKVKMEY--SKVEWPSRTEVIHSTLWVVTMTVLVSIYLGVFDILAVRA
LIsecE   -----MKVTTFIQECKAEL--EKVQWPTREEVVYSTVVVLVTVFFFSIFLFFADSAFVRL
TMsecE   ----MEKLRKFFREVIAEA--KKISWPSRKELLTSFGVVLVILAVTSVYFFVLDFIFSGV
ECsecE   LTTKGKATVAFAREARTEV--RKVIWPTRQETLHTTLIVAAVTAVMSLILWGLDGILVRL
         ••••,••••7••••,••••--8••••,••••9••••,••••10•••,••••11•••,•••

Supplemental Figure 1c (cont.)
         ----<
         HHHHHHHH
          a
         •6••••,••••7•••• 
MJs61g   ATYIKGILKPPTTPRV--------------
PHs61g   GILMLGG-----------------------
MetBs61g LTMLPQWVAQ--------------------
MKs61g   VQLIELYT----------------------
MTs61g   AQLLGG------------------------
FAs61g   MQIVF-------------------------
ThAs61g  YGCLFQDTLRWNPWKTARNMNGYRLTFQTA
AFs61g   MEILPGALK---------------------
PyAs61g  VIFSVLIYLMIKLR----------------
APs61g   YIILTSG-----------------------
SAs61g   TSLIIR------------------------
Hsps61g  MGGIVSVI----------------------
HSsec61g HIPINNIIVGG-------------------
Scsss1   HIPIRYVIV---------------------
BSsecE   IRLIVE------------------------
StrCsecE AKYVFG------------------------
AAsecE   ISFILSLRGSL-------------------
ChTsecE  MGKLL-------------------------
CTsecE   LSLFGKVTSFFFG-----------------
SyspsecE TKQPFLFG----------------------
TTsecE   IGLLR-------------------------
FNsecE   LNFLEALI----------------------
LIsecE   LTWFWELAG---------------------
TMsecE   VSAIFKALGIG-------------------
ECsecE   VSFITGLRF---------------------
         •12•••,••