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Highly Multiplexed Single-Cell RNA-seq for Defining Cell Population and Transcriptional Spaces

Gehring, Jase and Park, Jong Hwee and Chen, Sisi and Thomson, Matthew and Pachter, Lior (2018) Highly Multiplexed Single-Cell RNA-seq for Defining Cell Population and Transcriptional Spaces. . (Unpublished) http://resolver.caltech.edu/CaltechAUTHORS:20181030-145533155

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Abstract

We describe a universal sample multiplexing method for single-cell RNA-seq in which cells are chemically labeled with identifying DNA oligonucleotides. Analysis of a 96-plex perturbation experiment revealed changes in cell population structure and transcriptional states that cannot be discerned from bulk measurements, establishing a cost effective means to survey cell populations from large experiments and clinical samples with the depth and resolution of single-cell RNA-seq.


Item Type:Report or Paper (Discussion Paper)
Related URLs:
URLURL TypeDescription
https://doi.org/10.1101/315333DOIDiscussion Paper
ORCID:
AuthorORCID
Pachter, Lior0000-0002-9164-6231
Additional Information:The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license. bioRxiv preprint first posted online May. 5, 2018. We thank Valentine Svensson for helpful discussion during preparation of the manuscript. Paul Rivaud assisted with the data processing workflow.
Record Number:CaltechAUTHORS:20181030-145533155
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20181030-145533155
Official Citation:Highly Multiplexed Single-Cell RNA-seq for Defining Cell Population and Transcriptional Spaces. Jase Gehring, Jong Hwee Park, Sisi Chen, Matthew Thomson, Lior Pachter. bioRxiv 315333; doi: https://doi.org/10.1101/315333
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:90524
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:30 Oct 2018 22:35
Last Modified:30 Oct 2018 22:35

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