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Structural survey of HIV-1-neutralizing antibodies targeting Env trimer delineates epitope categories and suggests vaccine templates

Chuang, Gwo-Yu and Zhou, Jing and Rawi, Reda and Shen, Chen-Hsiang and Sheng, Zizhang and West, Anthony P., Jr. and Zhang, Baoshan and Zhou, Tongqing and Bailer, Robert T. and Doria-Rose, Nicole A. and Louder, Mark K. and McKee, Krisha and Mascola, John R. and Bjorkman, Pamela J. and Shapiro, Lawrence and Kwong, Peter D. (2018) Structural survey of HIV-1-neutralizing antibodies targeting Env trimer delineates epitope categories and suggests vaccine templates. . (Unpublished) https://resolver.caltech.edu/CaltechAUTHORS:20181030-152137214

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Abstract

HIV-1 broadly neutralizing antibodies are desired for their therapeutic potential and as templates for vaccine design. Such antibodies target the HIV-1-envelope (Env) trimer, which is shielded from immune recognition by extraordinary glycosylation and sequence variability. Recognition by broadly neutralizing antibodies thus provides insight into how antibody can bypass these immune-evasion mechanisms. Remarkably, antibodies neutralizing >25% of HIV-1 strains have now been identified that recognize all major exposed surfaces of the prefusion-closed Env trimer. Here we analyzed all 206 broadly neutralizing antibody-HIV-1 Env complexes in the PDB with resolution suitable to define their interaction chemistries. These segregated into 20 antibody classes based on ontogeny and recognition, and into 6 epitope categories (V1V2, glycan-V3, CD4-binding site, silent face center, fusion peptide, and subunit interface) based on recognized Env residues. We measured antibody neutralization on a 208-isolate panel and analyzed features of paratope and B cell ontogeny. The number of protruding loops, CDR H3 length, and level of somatic hypermutation for broadly HIV-1 neutralizing antibodies were significantly higher than for a comparison set of non-HIV-1 antibodies. For epitope, the number of independent sequence segments was higher (P < 0.0001), as well as the glycan component surface area (P = 0.0005). Based on B cell ontogeny, paratope, and breadth, the CD4-binding site antibody IOMA appeared to be a promising candidate for lineage-based vaccine design. In terms of epitope-based vaccine design, antibody VRC34.01 had few epitope segments, low epitope-glycan content, and high epitope-conformational variability, which may explain why VRC34.01-based design is yielding promising vaccine results.


Item Type:Report or Paper (Discussion Paper)
Related URLs:
URLURL TypeDescription
https://doi.org/10.1101/312579DOIDiscussion Paper
ORCID:
AuthorORCID
Bjorkman, Pamela J.0000-0002-2277-3990
Additional Information:The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. This article is a US Government work. It is not subject to copyright under 17 USC 105 and is also made available for use under a CC0 license. Posted May 2, 2018. We thank J. Stuckey for assistance with figures, and members of the Structural Biology Section and Structural Bioinformatics Core, Vaccine Research Center, for discussions or comments on the manuscript. We thank H. Mouquet and M. Nussenzweig for the nucleotide sequences of antibody 10-1074. We also thank J. Baalwa, D. Ellenberger, F. Gao, B. Hahn, K. Hong, J. Kim, F. McCutchan, D. Montefiori, L. Morris, J. Overbaugh, E. Sanders-Buell, G. Shaw, R. Swanstrom, M. Thomson, S. Tovanabutra, C. Williamson, and L. Zhang for contributing the HIV-1 envelope plasmids used in our neutralization panel. Support for this work was provided by the Intramural Research Program of the Vaccine Research Center, National Institute of Allergy and Infectious Diseases, and by IAVI Neutralizing Antibody Consortium (NAC).
Funders:
Funding AgencyGrant Number
National Institute of Allergy and Infectious Diseases (NIAID)UNSPECIFIED
IAVI Neutralizing Antibody ConsortiumUNSPECIFIED
Subject Keywords:antibody recognition, B cell ontogeny, broadly neutralizing antibody, cryo-electron microscopy, envelope glycoprotein trimer, glycan shielding, sequence variation, vaccine design, x-ray crystallography
Record Number:CaltechAUTHORS:20181030-152137214
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20181030-152137214
Official Citation:Structural survey of HIV-1-neutralizing antibodies targeting Env trimer delineates epitope categories and suggests vaccine templates. Gwo-Yu Chuang, Jing Zhou, Reda Rawi, Chen-Hsiang Shen, Zizhang Sheng, Anthony P. West Jr., Baoshan Zhang, Tongqing Zhou, Robert T. Bailer, Nicole A. Doria-Rose, Mark K. Louder, Krisha McKee, John R. Mascola, Pamela J. Bjorkman, Lawrence Shapiro, Peter D. Kwong. bioRxiv 312579; doi: https://doi.org/10.1101/312579
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:90526
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:30 Oct 2018 22:33
Last Modified:03 Oct 2019 20:26

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