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Molecular architecture, polar targeting and biogenesis of the Legionella Dot/Icm T4SS

Ghosal, Debnath and Jeong, Kwangcheol C. and Chang, Yi-Wei and Gyore, Jacob and Teng, Lin and Gardner, Adam and Vogel, Joseph P. and Jensen, Grant J. (2019) Molecular architecture, polar targeting and biogenesis of the Legionella Dot/Icm T4SS. Nature Microbiology, 4 (7). pp. 1173-1182. ISSN 2058-5276. PMCID PMC6588468. https://resolver.caltech.edu/CaltechAUTHORS:20181030-153432354

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Abstract

Legionella pneumophila survives and replicates inside host cells by secreting ~300 effectors through the defective in organelle trafficking (Dot)/intracellular multiplication (Icm) type IVB secretion system (T4BSS). Here, we used complementary electron cryotomography and immunofluorescence microscopy to investigate the molecular architecture and biogenesis of the Dot/Icm secretion apparatus. Electron cryotomography mapped the location of the core and accessory components of the Legionella core transmembrane subcomplex, revealing a well-ordered central channel that opens into a large, windowed secretion chamber with an unusual 13-fold symmetry. Immunofluorescence microscopy deciphered an early-stage assembly process that begins with the targeting of Dot/Icm components to the bacterial poles. Polar targeting of this T4BSS is mediated by two Dot/Icm proteins, DotU and IcmF, that, interestingly, are homologues of the T6SS membrane complex components TssL and TssM, suggesting that the Dot/Icm T4BSS is a hybrid system. Together, these results revealed that the Dot/Icm complex assembles in an ‘axial-to-peripheral’ pattern.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1038/s41564-019-0427-4DOIArticle
https://rdcu.be/byfVdPublisherFree ReadCube access
https://doi.org/10.1101/312009DOIDiscussion Paper
https://pdbj.org/emnavi/quick.php?id=emdb-0566Related ItemProtein Data Bank Japan
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6588468PubMed CentralArticle
ORCID:
AuthorORCID
Ghosal, Debnath0000-0002-2227-0330
Jeong, Kwangcheol C.0000-0003-4337-0426
Chang, Yi-Wei0000-0003-2391-473X
Vogel, Joseph P.0000-0002-8054-5021
Jensen, Grant J.0000-0003-1556-4864
Alternate Title:Molecular architecture of the Legionella Dot/Icm type IV secretion system
Additional Information:© 2019 The Author(s), under exclusive licence to Springer Nature Limited. Received 13 July 2018. Accepted 12 March 2019. Published 22 April 2019. We thank R. Isberg (Tufts University, Medford, MA, USA) for antibodies that recognize DotF and DotH, E. Buford for technical assistance and P. Levin (Washington University, St Louis, MO, USA) for assistance with deconvolution microscopy. ECT data were recorded at the Beckman Institute Resource Center for Transmission Electron Microcopy at Caltech and the cryo-EM facility at Janelia Research Campus. We thank C. Oikonomou for the creation of the domain maps and for help structuring and revising the text. We also recognize E. Darwin for key suggestions and critical appraisal of this manuscript. This work was funded by the NIH grant R01AI127401 to G.J.J. and the NIH grant R01AI48052 to J.P.V. These authors contributed equally: Debnath Ghosal, Kwangcheol C. Jeong. Author Contributions: D.G., K.C.C.J., J.P.V. and G.J.J. conceived the project. K.C.C.J., J.P.V. and J.G. constructed and characterized the L. pneumophila expression plasmids and strains. K.C.J. and J.P.V. collected the immunofluorescence data. D.G. collected the tomography data. D.G., K.C.C.J., J.P.V., G.J.J., Y.-W.C. and L.T. analysed the data. A.G. made Supplementary Video 1. D.G., J.P.V., K.C.C.J. and G.J.J. wrote the manuscript with input from other authors. Data availability: The subtomogram average of the Dot/Icm (DotF-sfGFP) complex that supports the findings of this study has been deposited in the Electron Microscopy Data Bank (EMDB) under the accession code: EMD-0566. All other density maps are available from the corresponding authors on request. The authors declare that all data supporting the findings of this study are available within the paper and its Supplementary Information documents. The authors declare no competing interests.
Funders:
Funding AgencyGrant Number
NIHR01AI127401
NIHR01AI48052
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Issue or Number:7
PubMed Central ID:PMC6588468
Record Number:CaltechAUTHORS:20181030-153432354
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20181030-153432354
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:90527
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:30 Oct 2018 22:39
Last Modified:17 Jan 2020 21:08

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